中药材2017，Vol.40Issue(5)：1026-1030,5.DOI:10.13863/j.issn1001-4454.2017.05.004
不同光质对桑黄的生长及代谢产物累积影响
Effects of Different Light Quality on the Growth and Metabolites Accumulation in Inonotus sanghuang
摘要
Abstract
Objective:To explore the effects of different light quality on the growth and metabolism accumulation in Inonotus sanghuang.Methods:Separation,purification and identification the wild fungus of Inonotus sanghuang,then the fungus was treated by dark,white,blue,green,yellow and red led light,the diameter,color change of the colony was recorded,and the change of the second metabolism and its free radical scavenging activity was analyzed by HPLC method and DPPH assay,respectively.Results:The fungus was identified as Inonotus sanghuang Sheng H.Wu,T Hatt.& Y.C.Dai by molecular technique.Different light quality showed significant influence on the mycelial growth.The dark,yellow and red light treatment were the similar growth speed and colony morphology.But the white,blue and green light treatment manifested remarkable growth inhibition effect,with sparse hyphae,but the colony was thick and the solid with a large amount of yellow pigment secretion.Meanwhile,HPLC analysis indicated that the white,blue and green light treated for 20 d could dramatically increase the type and the content of the second metabolisms(Hypholomine B and Inoscavin A)and enhance the DPPH radical scavenging activity.However,the intracellular polysaccharide was significatly decreased by blue light treatment;in contrast,the yellow light treatment significatly increased intracellular polysaccharide,and other treatment was similar with the dark treatment group.HPLC fingerprint profiles of the second metabolisms changed regularly according to the different light quality with different time treatment,which reconfirmed that the light with remarkable regulation effected on the metabolism of Inonotus sanghuang.Conclusion:Different light quality had important regulation effected on the growth,development and second metabolism accumulation in Inonotus sanghuang.Dark culture condition is beneficial to the mycelial growth and biomass increasing,the blue and green light can increase the active components accumulation in spite of the growth inhibition effect.Hence,further research should be taken to pave the way for scientifically and rationally exploring Inonotus sanghuang.关键词
桑黄/光质/次生代谢产物/高效液相色谱Key words
Inonotus sanghuang Sheng H.Wu ,T Hatt.& Y.C.Dai/Light quality/Second metabolism/HPLC分类
医药卫生引用本文复制引用
葛格,李美芽,薛章贤,尹芬芬,蓝巧帅,吕圭源,蒋福升..不同光质对桑黄的生长及代谢产物累积影响[J].中药材,2017,40(5):1026-1030,5.基金项目
浙江省大学生科技创新项目(2016R410036) （2016R410036）
