中药材2017，Vol.40Issue(5)：1056-1060,5.DOI:10.13863/j.issn1001-4454.2017.05.011
PCR-DGGE法分析不同产地蝉花真菌多样性
Analysis of Fungal Diversity of Isaria cicadae from Different Habitats by PCR-DGGE
刘腾飞 1史晓飒 1张洪梅 1张艳聪 1刘自尧 1刘元元 2史新元1
作者信息
- 1. 北京中医药大学中药学院,北京100029
- 2. 兰州理工大学,甘肃兰州730050
- 折叠
摘要
Abstract
Objective:To investigate the fungal diversity of Isaria cicadae from different habitats (Anhui,Jiangsu,Sichuan,Guangxi,etc.).Methods:PCR-DGGE (Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis) technology was adopted to analysis the fungal community structure.DGGE profiles showed that 16 specific bands were separated,these bands were cloned and sequenced.Results:lsaria cicadae from different habitats displayed significant difference in Shannon-Wiener index respectively.Community structure similarity was higher in Heyuan of Guangdong as well as Beichuan and mianyang of Sichuan.Conclusion:Cordyceps militaris is the dominant strains,and other Uncultured Wallemia,Uncultured eukaryote,Eurotium athecium,Magicicada cassini,Myrothecium roridum,Aspergillus penicillioides strains are also existed.关键词
蝉花/真菌多样性/18S rDNA/PCR-DGGEKey words
Isaria cicadae Miquel/Fungal diversity/18S rDNA/PCR-DGGE分类
医药卫生引用本文复制引用
刘腾飞,史晓飒,张洪梅,张艳聪,刘自尧,刘元元,史新元..PCR-DGGE法分析不同产地蝉花真菌多样性[J].中药材,2017,40(5):1056-1060,5.
