癌变·畸变·突变2017，Vol.29Issue(6)：418-421,426,5.DOI:10.3969/j.issn.1004-616x.2017.06.003
微囊藻毒素-LR对人正常食管上皮细胞凋亡及对Caspase-3和Caspase-9蛋白表达的影响
Effects of microcystin-LR on apoptosis, and Caspase-3 and Caspase-9 expression in human normal esophageal epithelial cells
摘要
Abstract
OBJECTIVE:To investigate the effects of microcystin-LR on viability and apoptosis of human normal esophageal epithelial cells,and on expression of Caspase-3 and Caspase-9.METHODS:Human normal esophageal epithelial cells were treated with microcystin-LR at different concentrations (1,3.75,7.5,15,30,50 μg/mL) for 24 h,and cell viability was detected by the MTT assay.The data were used to calculate the half inhibitory concentration (IC50) which was used to determine the MC-LR concentrations for subsequent experiments.Accordingly,cells were treated with 6,12 and 24 μg/mL MC-LR,and apoptosis was detected by PI-Annexin V double staining.Expression levels of Caspase-3 and Caspase-9 were detected by Western blot.RESULTS:Compared with the control group,microcystin-LR of 3.75-50 μg/mL decreased the viability of normal esophageal epithelial cells (P＜0.01);the 6-24 μg/mL concentrations increased the apoptosis rate (P＜O.05 or P＜O.01),and the expression of Caspase-3 and Caspase-9 (P＜0.01).CONCLUSION:Induction of apoptosis in human normal esophageal epithelial cells by microcystin-LR may be related to its activation of Caspase-3 and Caspase-9.关键词
微囊藻毒素-LR/人食管上皮细胞/凋亡/Caspase-3/Caspase-9Key words
microcystin-LR/human esophageal epithelial cells/apoptosis/Caspase-3/Caspase-9分类
医药卫生引用本文复制引用
肖红梅,吴逸,张婷,王少康,孙桂菊..微囊藻毒素-LR对人正常食管上皮细胞凋亡及对Caspase-3和Caspase-9蛋白表达的影响[J].癌变·畸变·突变,2017,29(6):418-421,426,5.基金项目
国家自然科学基金项目(81372985,81673147) （81372985,81673147）
