癌变·畸变·突变2017，Vol.29Issue(6)：422-426,5.DOI:10.3969/j.issn.1004-616x.2017.06.004
GMA诱导16HBE恶性转化细胞中LncRNAEMX2OS的表达及意义
Expression of LncRNA EMX2OS during glycidyl methacrylate-induced malignant transformation of 16HBE cells
摘要
Abstract
OBJECTIVE:To investigate the expression of long non-coding RNA EMX2OS (LncRNA EMX2OS) during glycidyl methacrylate (GMA)-induced malignant transformation of 16HBE cells.METHODS:Malignantly transformed 16HBE cells (induced by exposure to 8 μg/mL GMA) and solvent control cells (exposed to DMSO) at around the 30th generation were harvested.High throughput LncRNA microarrays were used to detect differences in expression profile of LncRNAs between the two groups of cells.Changes in LncRNAs were screened through strategies such as fold changes and neighboring genes analyses.Real-time fluorescence quantitative PCR (qPCR) and gene chip were applied to measure the expression levels of LncRNA EMX2OS and EMX2,respectively.RESULTS:Based on the result of LncRNA microarrays,expressions of LncRNA EMX2OS was up-regulated by 6.01 fold in the transformed cells campared to the control cells.In addition,qPCR analyses showed that LncRNA EMX2OS increased 3.37 fold and EMX2 increased 1.88 fold change (P＜0.05).The trends of increase in EMX2 and EMX2OS trends were identical.CONCLUSION:GMA induced significant increase in expression of LncRNA EMX2OS of 16HBE cells and the expression can be considered as a specific biomarker which is involved in GMA-induced malignant transformation 16HBE cells.关键词
甲基丙烯酸环氧丙酯/人支气管上皮细胞/LncRNA EMX2OS/EMX2Key words
glycidyl methacrylate/human bronchial epithelial cells/LncRNA EMX2OS/EMX2分类
医药卫生引用本文复制引用
王全凯,王博深,谢广云,康同影,朱宝立,许建宁..GMA诱导16HBE恶性转化细胞中LncRNAEMX2OS的表达及意义[J].癌变·畸变·突变,2017,29(6):422-426,5.基金项目
国家自然科学基金资助项目(81673221) （81673221）
