癌变·畸变·突变2017，Vol.29Issue(6)：431-436,6.DOI:10.3969/j.issn.1004-616x.2017.06.006
HPV16编码蛋白与宫颈癌细胞内CALCA和TFPI-2表达的关系
Expression of HPV16 coding proteins and regulation of CALCA and TFPI-2 expression in cervical carcinoma cells
摘要
Abstract
OBJECTIVE:We investigated the expression of CALCA and TFPI-2,and their association with expression of human papillomaviurs (HPV) 16 coding proteins in cervical carcinoma cells.METHODS:We designed and synthesized small-hairpin RNAs (shRNA) which were specific to the sequences of E6 or E7 genes of HPV16.In addition,we prepared a series of shRNA expression constructs using pRNAi plasmid that could simultaneously express shRNA and green fluorescent protein (GFP) reporter gene.After transient expression of shRNA vectors in HPV16-positive SiHa cervical carcinoma cells by lipid transfection,we analyzed transfection efficiency,inhibition of HPV-coding gene expression and its effect on candidate gene expression using fluorescent imaging and quantitative RT-PCR.RESULTS:Relatively high transfection efficiency was confirmed by using confocal microscopy on cell population based on their release of green fluorescence.According to quantitative RT-PCR analysis,E6 or E7 expressions were,to a very high extent,inhibited after transfection of shRNA vectors.Expressions of CDK4 and BCL-2 were significantly decreased after selective inhibition of E6 or E7 gene expression.These observations suggest that the inhibition of HPV16 coding gene expression might have inhibited cell proliferation and induced cell-cycle arrest.Expressions of CALCA and TFPI-2 were significantly upregulated after inhibition of E7 gene expression but not remarkable after inhibition of E6 gene expression.CONCLUSION:Results of this study together with previous reports suggest that HPV16 E7 protein may promote the downregulation of CALCA and TFPI-2 in cervical carcinoma cells.The mechanism may be involved with HPV-driven cervical carcinogenesis.关键词
HPV16/编码蛋白/SiHa细胞/宫颈癌/CALCA/TFPI-2Key words
HPV16/coding proteins/SiHa cells/cervical carcinoma/CALCA/TFPI-2分类
医药卫生引用本文复制引用
迪拉热·力迪甫,木塔力甫·艾买提,盛磊,古扎力努尔买提沙,艾尔肯·肉孜比拉力,ABULIZI Abudula..HPV16编码蛋白与宫颈癌细胞内CALCA和TFPI-2表达的关系[J].癌变·畸变·突变,2017,29(6):431-436,6.基金项目
新疆维吾尔自治区研究生教育创新计划科研创新项目(XJGRI2015071) （XJGRI2015071）
