癌变·畸变·突变2017，Vol.29Issue(6)：445-449,5.DOI:10.3969/j.issn.1004-616x.2017.06.008
DEHP对3β-羟类固醇脱氢酶表达水平的影响
Effects of di-(2-ethylhexyl)phthalate on expression of 3β-hydroxysteroid dehydrogenase
摘要
Abstract
OBJECTIVE:To study the effects of di-(2-ethylhexyl) phthalate (DEHP) on expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) in MCF-7 cells.METHODS:MCF-7 cells in cultured were exposed to DEHP in different doses (0.05,0.1,0.2,0.4,0.8 mmol/L) for 12,24,48 h,or to one dose (0.8 mmol/L) at different times (3,6,12,24,48 h).Expression of 3 β-HSD mRNA was detected by real-time PCR,expression of 3β-HSD protein was detected by western blot.RESULTS:After treatment of MCF-7 cells with DEHP at 0.1-0.8 mmol/L for 12-48 h,expression levels of 3β-HSD mRNA were significantly higher than that of the control group (P＜0.01).Treatment with the lowest dose of DEHP treatment (0.05 mmol/L) for 48h also induced significant increase of 3β-HSD mRNA expression (P＜0.05).Our study also show a dose-effect relationship.Expression of 3β-HSD mRNA also showed time-dependent effects after treatment with 0.8 mmol/L DEHP for 6,12,24,48 h (P＜0.01).Expression of 3β-HSD protein was also higher after treatment with DEHP at doses of 0.2-0.8 mmol/L (P＜0.05 or P＜0.01).In the 0.4,0.8 mmol/L dose group,expression of 3β-HSD protein level increased gradually with the increasing doses (P＜0.05 or P＜0.01).CONCLUSION:DEHP induced up-regulation of 3β-HSD mRNA and protein.Our findings indicate that the mechanism of DEHP toxicity may be related to the induction of changes of 3β-HSD during steroid synthesis.关键词
邻苯二甲酸二(2-乙基己)酯/3β-羟基类固醇脱氢酶/基因表达/生殖毒性Key words
di-(2-ethylhexyl) phthalate/3β-hydroxysteroid dehydrogenase/gene expression/reproductive toxicity分类
医药卫生引用本文复制引用
王利,彭鹏,徐新云,袁建辉,龙鼎新..DEHP对3β-羟类固醇脱氢酶表达水平的影响[J].癌变·畸变·突变,2017,29(6):445-449,5.基金项目
深圳市科技研发基础研究项目(JCYJ20150402102135509,JCYJ20170306160553495) （JCYJ20150402102135509,JCYJ20170306160553495）
