癌变·畸变·突变2017，Vol.29Issue(6)：450-453,4.DOI:10.3969/j.issn.1004-616x.2017.06.009
大鼠肝微粒体代谢对何首乌水提物肝细胞毒性的影响
Hepatotoxicity of aqueous extract of Polygoni Multiflori Radix with or without the rat liver microsome incubation
摘要
Abstract
OBJECTIVE:To study the hepatotoxicity of aqueous extract of Polygoni Multiflori Radix with or without incubation with the rat liver microsome.METHODS:Aqueous extract of Polygoni Multiflori Radix was incubated with or without rat liver microsome in vitro and then dried by freeze drying.Liver L02 and HepG2 cells were incubated with different concentrations of the freeze-dried extract (1.5,3,6,12 mg/mL) for 24 h,48 h.Cell vitability was assayed by MTT method.In addition,contents of three main components (trans-2,3,5,4'-tetrahydroxystibene-2-O-β-D-glucopyranoside,emodin-8-O-β-glucopy-ranoside,emodin) from aqueous extract of Polygoni Multiflori Radix after incubation were quantitated by HPLC analysis.RESULTS:The aqueous extract without incubation with rat liver microsome showed more toxicity at the concentration of 12 mg/mL than that with incubation (P＜0.01).Contents of the three main components of aqueous extract incubated with rat liver microsome were lower than those without rat liver microsome.CONCLUSION:Hepatotoxicity of aqueous extract of Polygoni Multiflori Radix was decreased after their metabolism by rat liver microsome.The relationship between reduced contents of the main components of the aqueous extract and the reduction of hepatotoxicity need further study for clarification.关键词
何首乌/水提物/肝微粒体/肝细胞毒性/代谢Key words
Polygoni Multiflori Radix/aqueous extract/liver microsome/hepatotoxicity/metabolism分类
医药卫生引用本文复制引用
吴双,李登科,李凯明,周明,孙震晓..大鼠肝微粒体代谢对何首乌水提物肝细胞毒性的影响[J].癌变·畸变·突变,2017,29(6):450-453,4.基金项目
国家自然科学基金项目(81473418) （81473418）
北京中医药大学研究生自主课题(2017-JYB-XS-145) （2017-JYB-XS-145）
