癌变·畸变·突变2017，Vol.29Issue(6)：454-459,6.DOI:10.3969/j.issn.1004-616x.2017.06.010
微小RNA-202对A549细胞增殖和周期的作用及机制
Regulatory mechanism of miR-202 on proliferation of A549 lung cancer cells
摘要
Abstract
OBJECTIVE:To investigate the regulatory mechanism on proliferation of miR-202 in A549 lung cancer cells.METHODS:Through the lentivirus transfection into A549 cells,up-and down-expression of miR-202 were investigated.DIANA-TOOLs and KEGG database were used to analyze the bioinformatic of miR-202 expression and to explore the signaling pathways that might be involved with potential target genes.Then,MTT assay and flow cytometry technology were applied to detect the influence of miR-202 on cell proliferation,apoptosis and cycle.RT-qPCR and Western blot methods were applied to detect the miR-202 regulation for its predicted target gene on both mRNA and protein levels.RESULTS:Bioinformatics analysis show that,miR-202 was involved in non-small cell lung cancer and other tumor-related pathways,and possibly in regulation of PIK3CA.Functional studies show that,compared with the negative control group,up-regulation of miR-202 inhibited cell proliferation (P＜0.05),caused cell cycle G1/S phase arrest (P＜0.05),but did not affect cell apoptosis (P＞0.05).Target gene study show that,compared with the control group,up-regulation of miR-202 reduced the expression of PIK3CA protein (P＜0.05),but had no effect on the expression of PIK3CA mRNA(P＞0.05).CONCLUSION:Expression of miR-202 influenced the proliferation and cycle of A549 cells through the negative regulation of PIK3CA and the study provides a new clue to the role and mechanism of miRNA in lung cancer.关键词
肺癌/miR-202/细胞增殖/细胞周期/调控机制Key words
Lung cancer/miR-202/cell proliferation/cell cycle/regulatory mechanism分类
医药卫生引用本文复制引用
彭慧,马书梅,洪伟伟,隋静,张艳秋,梁戈玉..微小RNA-202对A549细胞增殖和周期的作用及机制[J].癌变·畸变·突变,2017,29(6):454-459,6.基金项目
国家自然科学基金项目(81472939,81673132) （81472939,81673132）
江苏省科技厅2015年社会发展项目(BE2015719) （BE2015719）
南京市科技计划项目(201503006) （201503006）
