安徽农业科学2017，Vol.45Issue(36)：137-139,3.
适用于转录组测序的大豆根总RNA的提取方法研究
Study on Methods to Extract High-quality Total RNA from Soybean for RNA-Seq
摘要
Abstract
[Objective] To obtain total RNA from soybean roots with high quality,high yield and to meet the transcription group sequencing requirements.[Method]Three extracting effects were compared,including Trizol reagent,CTAB-LiCl method and Kit method.[Result] All three methods can extract total RNA from soybean.However,the extraction kit was the best with high purity and integrity RNA.The average concentration was 413.9 ng/μL,A260/A280 was approximately 2.14,A260/A230 was 2.10 by using extraction kit,which was repeatable.[Conclusion]The RNA extracted by Kit method meets the quality requirements for transcriptome sequencing and can be used for subsequent RNA-seq and other molecular biology-related studies.关键词
大豆/根/总RNA/转录组测序/提取方法Key words
Soybean/Root/Total RNA/RNA-seq/Extraction method分类
农业科技引用本文复制引用
谢钰珍,覃鸿妮,张宇,梅啸,吴凡..适用于转录组测序的大豆根总RNA的提取方法研究[J].安徽农业科学,2017,45(36):137-139,3.基金项目
苏州工业园区服务外包职业学院校级科研项目(ky-cyzx602). （ky-cyzx602）
