草业学报2017，Vol.26Issue(10)：77-86,10.DOI:10.11686/cyxb2016486
干旱胁迫下硅对肥皂草抗氧化系统及膜质稳定性的影响
Effects of silicon on the antioxidant system and membrane stability of Saponaria officinalis under drought stress
摘要
Abstract
The aim of this study was to determine the effect of silicon on the antioxidant system and membrane stability of Saponaria officinalis under drought stress.Silicon was added to soil at four concentrations (0.1,0.2,0.3,0.4 g/kg SiO2) and the soil moisture content was adjusted to impose normal conditions,mild drought stress,and severe drought stress on S.officinalis.The protective enzyme activity,antioxidant content,malondialdehyde (MDA) content,and relative electrical conductivity of the leaves of S.officinalis were determined.The results showed that with increasing severity and longer duration of drought stress,the activities of superoxide dismutase (SOD),peroxidase (POD),and catalase (CAT),and the contents of reduced glutathione (GSH) and ASA (ascorbic acid) in the leaves of S.officinalis first increased and then decreased,and the MDA content and relative electric conductivity increased.Compared with the control,the treatments with silicon added to soil showed higher activities of SOD,POD,CAT (＞30％ higher) under drought stress,and lower MDA content and relative electrical conductivity (＞25 ％ lower),resulting in more stable GSH and ASA contents.These results indicated that silicon can effectively alleviate damage to S.officinalis cell membranes under drought stress by improving the performance of the antioxidant system.As the concentration of silicon increased,the drought resistance of S.officinalis increased gradually and then stabilized.The optimal concentration of SiO2 in soil was 0.3-0.4 g/kg.关键词
硅/干旱胁迫/保护酶/抗氧化系统/细胞膜Key words
silicon/drought stress/protective enzyme/antioxidant system/the cell membrane引用本文复制引用
杨慧颖,邓雅楠,许凌欣,严俊鑫..干旱胁迫下硅对肥皂草抗氧化系统及膜质稳定性的影响[J].草业学报,2017,26(10):77-86,10.基金项目
东北油田盐碱植被恢复与重建教育部重点实验室(东北林业大学)开放基金(SAVER1609)和中央高校基本科研业务费专项基金(2572016CA11)资助. （东北林业大学）
