草业学报2017,Vol.26Issue(10):87-98,12.DOI:10.11686/cyxb2016502
干旱胁迫对高羊茅航天诱变新品系生理特性的影响及综合评价
Effects of drought stress on the physiological characteristics of new lines of Festuca arundinacea induced by spaceflight and their comprehensive evaluation
摘要
Abstract
In this study,the ecophysiological characteristics of 14 lines of Festuca arundinacea,including some induced by spaceflight,were studied in a pot experiment.The plants were subjected to drought stress conditions,and physiological and biochemical indices including soluble protein content,chlorophyll (Chl) content,and the activities of superoxide dismutase (SOD),peroxidase (POD),and catalase (CAT) activity were determined.These indexes of the 14 F.arundinacea were used to quantitatively evaluate their drought tolerance.The soluble protein content and Chl content increased,and the activities of SOD,POD,and CAT decreased under drought conditions.The drought resistance of the 14 F.arundinacea lines was calculated using the subordinate function,and the lines were ranked,from most drought tolerant to least drought tolerant,as follows:SP5-32>SP5-75>F.arundinacea cv.Shuicheng>SP5-60>SP5-94>SP5-97>SP5-89>SP5-71>SP5-7>SP5-85>SP5-42>SP5-5>F.arundinacea cv.Qiancao No.1>SP5-88.The average subordinate function of SP5-32,the most drought tolerant line,was 0.533.A grey correlative analysis was conducted to determine which indices were most strongly related to drought resistance.The indices were ranked,from strongest correlation with drought resistance to weakest,as follows:POD activity>soluble protein content>SOD activity>catalase activity>Chl content (correlation coefficients,0.55-0.65).Therefore,SP5-32 is more suitable for cultivation in drought-affected areas as its antioxidant enzyme activity,soluble protein content,and chlorophyll content are unaffected by drought stress.关键词
高羊茅/干旱胁迫/航天诱变/生理特性/综合评价Key words
Festuca arundinacea/drought stress/space mutation/physiological characters/comprehensive evaluation引用本文复制引用
李娟,雷霞,王小利,牟琼,杨春燕,吴佳海..干旱胁迫对高羊茅航天诱变新品系生理特性的影响及综合评价[J].草业学报,2017,26(10):87-98,12.基金项目
贵州省联合基金(黔科合J字LKN[2013]24号),贵州省重大专项(黔科合重大专项字[2014]6017号)和贵州省高层次创新型人才培养(黔科合人才[2016]4024号)资助. (黔科合J字LKN[2013]24号)