重庆医学2017，Vol.46Issue(34)：4766-4770,5.DOI:10.3969/j.issn.1671-8348.2017.34.004
β-TCP/PLGA复合BMSCs联合高压氧修复海水浸泡兔桡骨骨缺损的作用研究
Study on the effect of β-TCP/PLGA scaffolds seeded BMSCs combing with hyperbaric Oxygen on bone defect with seawater immersing in rabbit radius
摘要
Abstract
Objective To investigate therapeutic effects of β-TCP/PLGA scaffolds seeded bone marrow mesenchymal stem cells (BMSCs) combing with hyperbaric oxygen (HBO) on bone defect with seawater immersing in rabbit radius.Methods BMSCs were seeded into the β-TCP/PLGA scaffolds to construct tissue engineering bone.60 New Zealand rabbits were created 1.5 cm bone defect in bilateral radius,and then bilateral wound limbs were immersed in seawater for 3 h.After debridement,all rabbits were randomly divided into 4 groups.In group A nothing was implanted into the bone defect.Only BMSCs was implanted in group B.BMSCs + HBO was given in group C.β-TCP/PLGA BMSCs + HBO was given in group D.At postoperative 4,8,12 weeks rabbits were sacrificed after radiography radius.X-ray radiography,HE staining,immunohistochemical observation were used to evaluate repairing effect of bone defect with seawater immersing.Results Radiographic analysis demonstrated that the bone defects were completely repaired with recanalization of the medullary cavity in group D,bone defects were partially repaired with partal recanalization of the medullary cavity in group C,bone defects were incompletely repaired in group B;bone defect repair unfinished and the broken ends were sclerous in group A.Each time point group D＞group C＞group B＞group A in callus grey value (P＜0.05).HE staining indicated after 12 weeks,a small amount of lainellar bone formated in group A;a small amount of lamellar bone formated in group B;a large amount of lamellar bone formated in group C;a large amount of lamellar bone formated in group D.Immunohistoehemical result indicated at postoperative 4 weeks,the expressions of osteocalcin(OCN) in each group were at high levels,the expressions decreased significantly at Postoperative 8 weeks,the expressions were at a low level at postoperative 12 weeks.At postoperative 4 and 8 weeks,the expression levels of OCN were group D＞ group C＞ group B＞group A (P＜0.05),there was no significant difference at postoperative 12 weeks between the 4 groups (P＞0.05).Conelusion the β-TCP/PLGA composite BMSCs combined hyperbaric oxygen is the effective method to repair seawater immersed bone defect in rabbit.关键词
β-磷酸三钙/聚乳酸-聚羟乙酸/骨缺损/海水浸泡/高压氧/骨髓间充质干细胞Key words
β-TCP/PLGA/bone defect/seawater immersing/hyperbaric oxygen/BMSCs分类
医药卫生引用本文复制引用
张淦,程迅生,陈肖松,马武秀,陈聪聪,符来想..β-TCP/PLGA复合BMSCs联合高压氧修复海水浸泡兔桡骨骨缺损的作用研究[J].重庆医学,2017,46(34):4766-4770,5.基金项目
南京军区医学科技创新重点课题(11Z011). （11Z011）
