分析测试学报2017，Vol.36Issue(10)：1255-1259,5.DOI:10.3969/j.issn.1004-4957.2017.10.016
微波水解衍生高效液相色谱法测定饲料中的氨基酸
Determination of Amino Acids in Feed by High Performance Liquid Chromatography with Microwave Hydrolysis Derivatization
任晓宇 1裴晓静 1张少云 1谢立娜 1杜文博 1锁然1
作者信息
- 1. 河北农业大学食品科技学院,河北保定071000
- 折叠
摘要
Abstract
A high performance liquid chromatographic (HPLC) method with microwave hydrolysis derivation was developed for the simultaneous determination of 17 amino acids in feed.The method was based on pre-column derivatization with 2,4-dinitro-chlorobenzene.The compounds were separated on a C18 column.And the conditions of microwave hydrolysis were optimized.The detection was performed using a diode array detector at 360 nm.The calibration curves of 17 amino acids all exhibited good linearities in the range of 2.5-50 mg/L.The correlation coefficients(r2) for the determination varied between 0.990 7 and 0.999 9 with the relative standard deviations (RSDs) of 0.88％ to 4.2％,and the recoveries of 17 amino acids were in the range of 90.6％-107.2％ with the limits of detection of 0.15-2.37 mg/L.The results showed that the results of microwave hydrolysis in 16 min at 150 ℃ are basically the same as those of traditional heating hydrolysis(110 ℃,24 h).With the advantages of short analysis time,high sensitivity,the method could be used for the determination of amino acids in feed.关键词
微波水解/氨基酸/高效液相色谱法/柱前衍生/饲料Key words
microwave hydrolysis/amino acid/high performance liquid chromatography (HPLC)/pre-column derivatization/feed分类
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任晓宇,裴晓静,张少云,谢立娜,杜文博,锁然..微波水解衍生高效液相色谱法测定饲料中的氨基酸[J].分析测试学报,2017,36(10):1255-1259,5.
