南方农业学报2017，Vol.48Issue(9)：1653-1658,6.DOI:10.3969/j.issn.2095-1191.2017.09.19
复硝酚钠与GA3复配对美国红栌组培苗生长的影响
Effects of combination sodium nitraphenolate+GA3 on Cotinus coggygria tissue culture plants
摘要
Abstract
[Objective] Effects of plant growth regulator combination sodium nitraphenolate+ gibberellin (GA3) at different concentrations on the growth of Cotinus coggygria were explored in order to provide technical support for shortening the culture cycle of tissue culture plants.[Method]B5 medic adding sodium nitraphenolate+GA3 of different combinations were used to culture C.coggygria.Leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number were investigated after 25 d of culture to study the effects of adding sodium nitraphenolate+GA3 on the tissue culture plants.[Result] 0.020 mg/L sodium nitraphenolate+30.0 mg/L GA3 had the optimal effects on C.coggygria tissue culture plants.Leaf number per plant,plant height,fresh weight,dry weight were significantly higher than those in control (CK) and other treatments (P＜0.05).Leaf number per plant,plant height,fresh weight,dry weight increased 83.3％、71.8％、86.6％ and 85.8％ respectively compared with CK.Rooting was also better than CK and the highest rooting rate reached 95.2％.The plants were strong with large root number,thick leaves and sound growth state.Correlation analysis indicated that there was extremely positive correlation between nitraphenolate+GA3 concentrations and leaf number per plant,plant height,fresh weight,dry weight,rooting rate and root number(P＜0.01).[Conclusion]Adding 0.020 mg/L sodium nitraphenolate+30.0 mg/L GA3 into B5 medium can improve the growth for C.coggygria tissue culture plants,and shorten the culture cycle and speed up the breeding process.关键词
美国红栌/组培苗/复硝酚钠/赤霉素(GA3)/复配Key words
Cotinus coggygria/tissue culture plant/sodium nitraphenolate/gibberellin(GA3)/combination分类
农业科技引用本文复制引用
杨艳敏,贾海丽,潘一展..复硝酚钠与GA3复配对美国红栌组培苗生长的影响[J].南方农业学报,2017,48(9):1653-1658,6.基金项目
河南省重点科技攻关项目(13210211139) （13210211139）
河南省高等学校青年骨干教师项目(2016GGJS-180) （2016GGJS-180）
商丘市重点科技攻关项目(153021) （153021）
商丘学院科研创新团队项目(170517) （170517）
