不同成花量金花茶花果期果枝叶内源激素的变化
Dynamic changes of endogenous hormones in fruit branch leaves of Camellia nitidissima with different flower numbers during blossom and fruit periods
摘要
Abstract
In order to investigate the physical problem of flowering and fructification rate of endangered plant Camellia nitidissima,and provide the theory basis for species conservation,the contents and ratio of GA3,IAA,ZR and ABA in fruit branch leaves of C.nitidissima of more-flower,less?flower and non?flower were measured by ELISA(Enzyme?linked immunosorbent assay)method during blossom and fruit periods.In early?bloom stage,the content of IAA in leaves rised firstly and then fell,and its content of more?flower?plant was lower than that of less?flower?plant leaves;the content of ZR in flower?plant was higher than that of non?flower?plant;the content of GA3 appeared an upward trend,and its content of flower?plant was higher than non?flower?plant;the contents of ABA decreased firstly and then increased,and its content of more?flower?plant was lower than non?flower?plant. In full?bloom stage, the contents of IAA, GA3and ABA declined overall;the content of ZR rised firstly and then fell;the contents of IAA,ZR,GA3in more?flower plant were higher than those in less?flower?plant or non?flower?plant, while the content of ABA was less than that of other plants. During the bloom period,the ratio of IAA/ZR,IAA/ABA,ZR/ABA and GA3/ABA of flower?plant were higher than that of non?flower?plant,but the ratio of(IAA+GA3)/ZR was just in contrast,which also indicate the development of flower and fruit was related to not only single plant hormone but also the balance of plant hormones. During autumn shoot period with active vegetative growth,the ratio of IAA/ZR of non?flower?plant was larger than that of flower?plant, while the ratio of IAA/ZR of non?flower?plant was smaller in blossom and fruit period with vigorous reproductive growth, but the ratio of(IAA+GA3)/ZR was just in contrast.The results showed that high level of IAA,ABA,ZR and low level of GA3in bud stage was benefitial for blossom,high content of IAA,ZR and low content of GA3, ABA in post?bloom stage was conducive to reducing blossom and fruit dropping, increased fruit?bearing rate and promoting fruit rapid growth,high ABA content before harvesting was favorable toward fruit ripening.Therefore,it can provide theoretical reference for using plant growth regulator to reduce the fallen petal and fruit abscission in production of C. nitidissima.关键词
金花茶/果枝叶/成花量/花果期/吲哚乙酸/赤霉素/玉米素核苷/脱落酸Key words
Camellia nitidissima/fruit branch leaves/flower number/blossom and fruit periods/IAA/GA3/ZR/ABA分类
农业科技引用本文复制引用
孙红梅,廖浩斌,刘盼盼,杨雪,李吉涛,漆小雪..不同成花量金花茶花果期果枝叶内源激素的变化[J].广西植物,2017,37(12):1537-1544,8.基金项目
国家自然科学基金(31660092) （31660092）
广西自然科学基金(2015GXNSFBA139051, 2016GXNSFAA380122) （2015GXNSFBA139051, 2016GXNSFAA380122）
广西植物研究所基本业务费项目(桂植业14004,17006) （桂植业14004,17006）
桂林市科技攻关项目(20140115?1) （20140115?1）
柳州市科技攻关项目(2016B050202) （2016B050202）
广西植物功能物质研究与利用重点实验室主任项目(ZRJJ2016?21)[Supported by the National Natural Science Foundation of China(31660092) （ZRJJ2016?21）
the Natural Science Foundation of Guangxi(2015GXNSFBA139051, 2016GXNSFAA380122) （2015GXNSFBA139051, 2016GXNSFAA380122）
Fundamental Research Foundation of Guangxi Institute of Botany(14004,17006) （14004,17006）
the Key Scientific and Technological Program of Guilin(20140115?1) （20140115?1）
the Key Scientific and Technological Program of Liuzhou(2016B050202) （2016B050202）
the Fund of Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization(ZRJJ2016?21)]. （ZRJJ2016?21）
