湖南中医药大学学报2017，Vol.37Issue(12)：1320-1322,3.DOI:10.3969/j.issn.1674-070X.2017.12.006
猴头菌多糖对Lewis肺癌荷瘤小鼠抑瘤作用及其机制研究
Study of the Inhibition and Its Possible Mechanisms of Hericium Polysaccharides on Mice with Lewis Lung Cancer
摘要
Abstract
Objective To study the inhibition and its possible mechanisms of Hericium polysaccharides (HPS) on mice with Lewis lung cancer. Methods 50 mice were chosen to establish Lewis lung cancer mice models, and were divided randomly into five groups: HPS high-dose group, HPS middle-dose group, HPS low-dose group, cyclophosphamide (CTX) group and model control group, 10 mice in each group. The mouse in former three groups were given HPS 200 mg/kg, 100 mg/kg and 50 mg/kg daily for 10 days, respectively, while those in CTX group were given CTX 40 mg/kg daily for 3 days, and those in model control group were given physiological saline 0.1mg/10g daily for 10 days. Another 10 healthy mice were chosen as the normal control group. The blood from eyeball of mice were collected, and the blood serum were separated, and tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2) were detected by ELISA. The tumor inhabitation rates were calculated. Results Compared with model control group, the tumor weights in CTX group and HPS high- dose, middle- dose and low- dose group all significantly decreased (P<0.05) with tumor inhabitation rates 73.52％, 44.33％, 29.65％ and 16.58％, which had statistical significance between each group (P<0.05). The level of TNF-α was higher and the level of IL-2 in model control group was lower than those in normal control group (P<0.05). Compared with model control group, the level of TNF-α significantly increased and the level of IL-2 significantly decreased in CTX group and HPS groups (P<0.05). The change trends of CTX group were more obvious than those in HPS groups (P<0.05), while there was no significant differences among HPS different dose groups (P>0.05). Conclusion HPS has dose-dependent inhibitory effect on mice with Lewis lung cancer, which may be associated with improvement of serum TNF-α and IL-2 level, thus improving immune function.关键词
猴头菌多糖/Lewis肺癌/TNF-α/IL-2Key words
Hericium polysaccharide/Lewis lung cancer/TNF-α/IL-2分类
医药卫生引用本文复制引用
周辉,刘畅,刘杨霖,张琦,游玉宇,欧慧,徐康平,肖玲..猴头菌多糖对Lewis肺癌荷瘤小鼠抑瘤作用及其机制研究[J].湖南中医药大学学报,2017,37(12):1320-1322,3.基金项目
2017年度湖南省中医药科研计划项目(201768) （201768）
湖南省财政厅项目[湘财社指(2016)129号] （2016）
湖南省自然科学基金项目(2015JJ6058). （2015JJ6058）
