中医药导报2017，Vol.23Issue(21)：82-85,4.
肾气丸对2型糖尿病阳虚证大鼠骨骼肌线粒体呼吸链复合体及ATP酶活性的影响
Effects of Shenqi Pill (肾气丸) on the Respiratory Chain Complexes and ATP Enzyme Activity of the Skeletal Muscle Mitochondria in Type Ⅱ Diabetic Rats with Yang Deficiency Syndrome
摘要
Abstract
Objective:To study the effects of Shenqi Pill on the respiratory chain complexes and ATP enzyme activity of the skeletal muscle mitochondria in type Ⅱ diabetic rats with yang deficiency syndrome.Methods:A total of 60 Sprague-Dawlcy rats were randomly divided into normal group (12 rats) and model group (48 rats),and the 48 SD rats were randomly divided into model group,Shenqi Pill group,Bu Yang group and rosiglitazone group after used high-sugar and high-fat feed 3 weeks and small dose streptozotocin and hydrocortisone 2 weeks to induce type 2 diabetic rats with yang deficiency syndrome.The rats were killed after 3 weeks administration to measure the mitochondrial respiratory chain complex and ATP enzyme activity of the skeletal muscle by enzyme linked immunosorbent assay (ELISA).Results:The activity of Na+-K+-ATPase,Ca2+-Mg2+-ATPase and mitochondrial respiratory chain complex Ⅰ,Ⅱ,Ⅲ,Ⅳ in the model group were lower than the normal group(P＜0.01).The activity of Na+-K+-ATPase,Ca2+-Mg2+-ATPase and mitochondrial respiratory chain complex Ⅰ,Ⅱ,Ⅲ,Ⅳ in Shenqi Pill and rosiglitazone group was significantly higher than model group (P＜0.05).However,there was no statistically significant difference between the two groups.The activity of Na+-K+-ATPase,Ca2+-Mg2+-ATPase and mitochondrial respiratory chain complex Ⅰ,Ⅱ,Ⅲ,Ⅳ in the Bu Yang group was significantly lower than Shenqi Pill group (P＜0.01).Conclusion:Shenqi Pill can improve the respiratory chain complexes Ⅰ,Ⅱ,Ⅲ,Ⅳ and ATP enzyme activity of the skeletal muscle mitochondria in type Ⅱ diabetic rats with yang deficiency syndrome.关键词
肾气丸/呼吸链复合体/ATP酶/大鼠Key words
Shenqi Pill/Respiratory Chain Complexes/ATP Enzyme/Rats分类
医药卫生引用本文复制引用
李美红,邱林,赵群菊,邓娜,刘仙菊,胡方林..肾气丸对2型糖尿病阳虚证大鼠骨骼肌线粒体呼吸链复合体及ATP酶活性的影响[J].中医药导报,2017,23(21):82-85,4.基金项目
湖南省自然科学基金(2015JJ2110) （2015JJ2110）
湖南省中医药管理局资助基金(重点)(201437) （重点）
湖南省中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(ZYFT201409) （ZYFT201409）
