华侨大学学报（自然科学版）2017，Vol.38Issue(6)：768-773,6.DOI:10.11830/ISSN.1000-5013.201703056
光学显微系统的图像质量优化方法
Image Quality Optimization Method of Optical Microscopy System
摘要
Abstract
The condenser of a transmissive optical microscope was optimized for improving its illumination uniformity and intensity.First,the adverse impact on the image quality resulting from potential misalignment of optical axes and small aperture of a multi-lens Abbe condenser was discussed.Then,an optimized design was proposed by using a single aspheric lens to replace the multi-lens Abbe condenser to improve the uniformity and intensity of illumination system.Finally,experiments were conducted to investigate the effects of the single aspherie lens condenser on image quality.The experimental results showed that the illumination uniformity of the microscope with the optimized condenser reached 0.50％ and 0.66％ under objectives with 20X and 40X magnifications respectively which were about 5 times better than those of the two-spherical lens condenser.Meanwhile,the intensity of the microscope with the new condenser increased over 15％ and 17％ under 20X and 40X objective lenses respectively as compared to those of the two-lensed system.Therefore,the optimized illumination system with a single aspheric lens can effectively improve the image uniformity and the intensity of the microscope,which results in an image of better quality.关键词
照明均匀度/光照度/非球面透镜/阿贝聚光镜/光学显微成像方法Key words
illumination uniformity/illumination intensity/aspheric lens/abbe condenser/optical microscope imaging method分类
信息技术与安全科学引用本文复制引用
崔长彩,张勇贞,易定容,孔令华,刘志群..光学显微系统的图像质量优化方法[J].华侨大学学报（自然科学版）,2017,38(6):768-773,6.基金项目
国家自然科学基金资助项目(51235004,51475176) （51235004,51475176）
