海洋科学2017，Vol.41Issue(8)：24-31,8.DOI:10.11759//hykx20161202001
一株海洋细菌YCSC6的鉴定及其体外杀盾纤毛虫的能力
Identification of marine bacterium YCSC6 and its insecticidal ability against Scuticociliate in vitro
摘要
Abstract
To develop a safe and effective new drug for the prevention and control of turbot scuticociliate disease, from the library of offshore marine microbial resources we screened out a marine bacterium that showed insecti-cidal activity against scuticociliate in vitro. We identified YCSC6 as Salinivibrio costicola using morphological and molecular biological methods. We conducted co-culture pharmacodynamics experiments in which YCSC6 killed pathogenic scuticociliate. We characterized the insecticidal properties of YCSC6 by optical and scanning electron microscopy. The results show that the fermentation supernatant of YCSC6 can lead to membrane perforation in scuticociliate, after which the scuticociliate loses its membrane integrity and cracks. After a certain period of time, the cracking ability strengthens with time. In this research, we made preliminary explorations of the insecti-cidal characteristics and ability in vitro of YCSC6 and found it to be a safe and effective source for the development of a microbe insecticide for aquaculture.关键词
大菱鲆(Scophthalmusmaximus)/盾纤毛虫(scuticociliate)/YCSC6/裂解/微生物杀虫剂Key words
turbot/scuticociliatlda/YCSC6/lytic process/microbe insecticide分类
生物科学引用本文复制引用
杜光迅,曲凌云,尚琨,王琛,高萍..一株海洋细菌YCSC6的鉴定及其体外杀盾纤毛虫的能力[J].海洋科学,2017,41(8):24-31,8.基金项目
十三五海洋环境安全保障重点专项(2017YFC1404504) （2017YFC1404504）
山东省自主创新及成果转化专项(2014ZZCX06205) （2014ZZCX06205）
青岛海洋科学与技术国家实验室鳌山科技创新计划(2015ASKJ02-03) [National Key R & D Program of China, No. 2017YFC1404504 （2015ASKJ02-03）
Independent Innovation and Achievement Transformation Project in Shandong Province, No. 2014ZZCX06205 （）
The Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology, No. 2015ASKJ02-03] （）
