减数分裂期雄性大菱鲆孕激素及受体基因的表达分析
Progestin actions and expression analysis of progestin receptors in the meiotic in male turbot (Scophthalmus maximus)
摘要
Abstract
To explore the function of progestin during spermatogonial proliferation and meiosis, we used histological methods to investigate the gonadal development process of the male turbot (Scophthalmus maximus) 6 to 22 months of age. We detected the expression of amh and sycp3 via the real-time quantitative polymerase chain reaction (qRT-PCR). Using enzyme-linked immunosorbent (ELISA) technology, qRT-PCR, and in-situ hybridization, we detected the serum P4 and DHP levels and the expression patterns of the nuclear progesterone receptor (pgr) and membrane progestin receptor alpha (mPRa) during different development periods. The results reveal that the P4 and DHP levels were high during spermatogonial proliferation and meiosis Ⅱ. The pgr expression was high from spermatogonial proliferation to meiosis I, whereas the mPRα expression was high during meiosis Ⅱ and spermiogenesis. Our in-situ hybridization results indicate pgr mRNA to be predominantly located in the Sertoli cells that were in contact with the proliferating spermatogonia and primary spermatocyte and that the mPRα mRNA was undetectable. Taken together, our data indi-cate the pgr expression to be involved in mediating the progestin stimulation of spermatogonia proliferation and meio-sis, whereas the mPRa plays an important role in meiosis Ⅱ and spermiogenesis.关键词
大菱鲆(Scophthalmusmaximus)/减数分裂/孕激素/pgr/mPRαKey words
Scophthalmus maximus/meiosis/progestin/pgr/mPRα分类
生物科学引用本文复制引用
丰程程,柳意樊,安皓,徐世宏,王彦丰,宋宗诚,刘清华,李军..减数分裂期雄性大菱鲆孕激素及受体基因的表达分析[J].海洋科学,2017,41(8):91-98,8.基金项目
国家自然科学基金资助项目(31472264, 31572602) （31472264, 31572602）
鳌山科技创新计划资助项目(2015ASKJ02, 2015ASKJ02-03-03)资助 （2015ASKJ02, 2015ASKJ02-03-03）
鲆鲽类产业技术体系项目(nycytx-50) （nycytx-50）
中国科学院青促会项目(KFJ-EW-STS-060) （KFJ-EW-STS-060）
国家科技基础条件平台建设运行项目(2016DKA30470) [National Science Foundation of China, No. 31472264, 31572602 （2016DKA30470）
The Scientific and Technological Innovation Project Finan-cially Supported by Qingdao National Laboratory for Marine Science and Technology, No.2015ASKJ02, 2015ASKJ02-03-03 （）
Modern Agro-Industry Technology Research System, No.nycytx-50 （）
Youth Innovation Promotion Association CAS and Chinese Academy of Science and Technology Ser-vice Network Planning, No.KFJ-EW-STS-060] （）
Project from the National Infrastructure of Fishery Germplasm Resource, No.2016DKA30470]] （）
