海洋科学2017，Vol.41Issue(8)：99-107,9.DOI:10.11759/hykx20161222001
一株海藻多糖降解菌的分离鉴定及产酶条件优化
Isolation identification and enzyme-producing conditions analysis of a seaweed polysaccharides-degrading bacteria
摘要
Abstract
In this study, we isolated a marine agar and alginate-degrading Gram-negative bacterial strain ST-6 from brown seaweed. The results of our 16S rDNA sequence analysis indicate that the similarity was 99％ between the strain ST-6 and the strains of Pseudomonas pachastrellae. We classified these strains as a class in phylogenetic trees using the NJ method and identified the strain as P. pachastrellae ST-6. After culturing in a 2216E medium at 30℃, we simultaneously analyzed the biomass accumulation and enzyme production of P. pachastrellae ST-6. The growth curve results indicate the exponential and stationary growth phases to be 10-48 h and 48-72 h after inoculation, respectively. The enzyme production results indicate that the enzyme activity was higher in the exponential growth phase and that the highest extracelluar agarase relative activity was 249.15 U/mL 48 h after inoculation. In addition, the secretion types of agarase and alginate lyase of P. pachastrellae ST-6 were non-inducible and inducible, respec-tively. The optimum growth conditions were a culture temperature of 25-35℃ and initial pH values of 5 and 9, respectively. The optimum agarase-producing conditions were a culture temperature of 30℃, an initial pH value of 7, and an initial agar concentration of 0.30％. The optimum alginate lyase-producing conditions were a culture temperature of 35℃, an initial pH value of 9, and an alginate concentration of 0.30％. We obtained a maximum relative extracelluar alginate lyase activity of 135.54 U/mL in culture conditions with a temperature of 30℃, a pH value of 7, and an alginate concentration of 0.30％.关键词
海绵假单胞菌/琼胶酶酶活/褐藻胶酶酶活/3,5-二硝基水杨酸比色法/产酶条件优化Key words
Pseudomonas pachastrellae/agarase activity/alginate lyase activity/3/5-Dinitrosalicylic acid colorimetry method/condition optimization of enzyme production分类
农业科技引用本文复制引用
朱大玲,唐啸龙,张宝玉,杨振芳,王乐普,秦乐宁,张蕾..一株海藻多糖降解菌的分离鉴定及产酶条件优化[J].海洋科学,2017,41(8):99-107,9.基金项目
青岛海洋科学与技术国家实验室开放基金项目(0F2015N015) （0F2015N015）
天津科技大学青年教师创新基金项目(2015LG10) （2015LG10）
天津市科技支撑计划项目(15ZXCXSF00040) （15ZXCXSF00040）
国家自然科学基金项目(21406169) [the Open Fund of Laboratory for Marine Biology and Bio-technology, Qingdao National Laboratory for Marine Science and Tech-nology, No. 0F2015N015 （21406169）
the Youth Innovation Foundation of Tianjin University of Science and Technology, No. 2015LG10 （）
the Key Technolo-gies R & D Program of Tianjin, No. 15ZXCXSF00040 （）
National Nature Science Foundation, No. 21406169] （）
