海洋科学2017，Vol.41Issue(8)：108-115,8.DOI:10.11759/hykx20160930001
Hg对锦州湾表层沉积物异养细菌代谢酶活性的影响研究
Research on effects of Hg on the metabolic enzymes of het-erotrophic bacteria in surface sediments of Jinzhou Bay
摘要
Abstract
To understand the effects of Hg on the major metabolic enzymes of heterotrophic bacteria in the surface sediments of Jinzhou Bay, in this study, we conducted field investigations and collected surface sediment samples from Jinzhou Bay in August 2012. We evaluated the potential ecological risk of Hg using the Hakanson method and performed diffusion plate assays to measure the activity of four kinds of metabolic enzymes of heterotrophic bacte-ria. The results showed that the content and potential ecological risk of Hg were highest and most serious in the estuary and this situation gradually weakened from the estuary to the ocean, which indicated that most Hg in the surface sediments of Jinzhou Bay originated from land sources. The metabolic enzyme activity of heterotrophic bacteria and the ratio of resistant heterotrophic bacteria were both higher in the southwest coastal waters than in the northeast offshore waters, which were also similar to the distribution of the heavy metal Hg content and the Hakanson potential ecological risk. This indicates that the metabolism of heterotrophic bacteria changed under long-term high concentrations of heavy metals pollution and produced a certain physiological resistance to heavy metals. The contents of phosphatase, protease, and lipase showed a significant positive correlation with the content of the heavy metal Hg, respectively, which indicates that they can be used as sensitivity indexes to reflect heavy metal Hg pollution in the surface sediments of Jinzhou Bay.关键词
重金属/潜在生态危害/异养细菌/水解酶/平板扩散法/抗性Key words
heavy metal/potential ecological risk/heterotrophic bacteria/metabolic enzyme/diffusion plate assay/resistance分类
资源环境引用本文复制引用
徐庆彤,刘瑞志,郭小雅,李捷,雷坤,刘庆庆,郝晨林..Hg对锦州湾表层沉积物异养细菌代谢酶活性的影响研究[J].海洋科学,2017,41(8):108-115,8.基金项目
科技基础工作专项(2013FY111100-005) （2013FY111100-005）
海洋公益性行业科研专项项目(201505001) （201505001）
山东省自然科学基金项目(ZR2013DM003) （ZR2013DM003）
环保公益性行业科研专项(201409100) [National Science and Technology Basic Work, No.2013FY111100-005 （201409100）
Special Fund for Marine Public Welfare Industry Research, No.201505001 （）
Natural Science Foundation of Shandong Province, No. ZR2013DM003 （）
Special Fund for Environmental Public Welfare Industry Research, No.201409100] （）
