海洋科学2017，Vol.41Issue(8)：116-121,6.DOI:10.11759/hykx20161023002
中心硅藻梅尼小环藻淡水株和海水株亚显微结构的形态学差异
Ultrastructure of the centric diatom Cyclotella meneghiniana:morphological differences between freshwater and seawater strains
摘要
Abstract
In this paper, four strains were identified using 18S rRNA gene sequences. The results show that the 18S rRNA gene sequences of these four strains (two come from freshwater and two from seawater) are very similar and that these four strains are closely related to Cyclotella meneghiniana (GQ148712 and AY496212) with high support (99/98 for neighbor joining/maximum likelihood). According to the molecular results, the four strains are of the same species, i.e., C. meneghiniana. Then, the ultrastructure of the four strains was studied using scanning electron microscopy (SEM). The results show that the ultrastructure of freshwater C. meneghiniana is different from that of seawater C. meneghiniana, which also have larger cells and contain higher amounts of central fultoportula. In addi-tion, this paper analyzed the causes of high-level morphology differences within C. meneghiniana species and dis-cussed whether the 18S rRNA gene can be used as a molecular marker for the identification of Cyclotella species.关键词
硅藻/梅尼小环藻(Cyclotellameneghiniana)/亚显微结构/扫描电镜/18SrRNAKey words
diatom/Cyclotella meneghiniana/ultrastructure/scanning electron microscopy/18S rRNA分类
生物科学引用本文复制引用
李长玲,陈健冰,黄翔鹄..中心硅藻梅尼小环藻淡水株和海水株亚显微结构的形态学差异[J].海洋科学,2017,41(8):116-121,6.基金项目
广东省海洋渔业科技与产业发展专项(A201508B08) [Marine Fishery Science and Technology and Industry De-velopment Project of Guangdong Province, No.A201508B08] （A201508B08）
