家畜生态学报2017，Vol.38Issue(11)：15-20,6.
基于RNA-Seq绒山羊皮肤两种毛囊差异表达基因的分析
Analysis on Differentially Expressed Genes by RNA-Seq in Two Kinds of Hair Follicle in Cashmere Goat's Skin
摘要
Abstract
This research is conducted to screen differentially expressed genes (DEGs) between the primary and secondary hair follicle (HF) of Cashmere goat's skin to find candidate genes related to development.To analyze the transcriptome data by bioinformatics methods,the RNA-Seq of six individuals from Inner Mongolia Arbas Cashmere goat was performed.On the basis of quality control of the sequencing result,DEGs were identified as well as functional annotation and enrichment analysis was carried out.In this study,617 DEGs has been selected from primary and secondary HF,and among them 297 are up-regulated while 320 are down-regulated.Additionally,the results of gene ontology (GO) expression elucidate that the main function of up-regulated genes are:electron transport chain,respiratory chain electron transport,mitochondrial electron transport,gene expression and other biological functions.However down-regulated expression of genes is mainly exercised as following:cell autophagy,protein complex,neutrophil aggregation,bacterial fungal defense reaction and other biological functions.Using KEGG database as a reference,these genes are mainly involved in the metabolism of cysteine and methionine,RNA aggregation,shear,Parkinson's disease,substrate phosphorylation,and Alzheimer's disease.It will provide a basis for further study on the related genes and functions of hair follicle development in the future.关键词
绒山羊/毛囊/转录组/RNA-测序Key words
cashmere goat/hair follicle/transcriptome/RNA-sequencing分类
农业科技引用本文复制引用
苏蕊,张燕军,李金泉,乔贤,范一星,李晓凯,张磊,马宇浩,王瑞军,王志英,刘志红..基于RNA-Seq绒山羊皮肤两种毛囊差异表达基因的分析[J].家畜生态学报,2017,38(11):15-20,6.基金项目
国家绒毛用羊现代农业产业技术体系项目(CARS-39-06) （CARS-39-06）
国家自然科学基金(31660639) （31660639）
