生产单一性别羔羊的奶山羊卵巢组织差异表达microRNAs筛选OA北大核心CSTPCD
Differentially Expressed MicroRNAs Screening in Ovaries of Dairy Goat Producing Same-Sex Lambs
运用Solexa/Illumina高通量测序技术分析生产单一性别羔羊的奶山羊卵巢miRNAs表达情况,探讨miRNAs在繁殖调控中的作用.采集4～6岁始终生产单一性别羔羊的奶山羊母羊双侧卵巢,提取总RNA,建立cDNA文库,并进行克隆、测序及生物信息学分析.结果表明,在单一产公羔奶山羊(ASGO1)卵巢中有744 782个reads,包含127 205条miRNAs.单一产母羔奶山羊(ASGO2)中有1 602 751个reads,包含454 9…查看全部>>
The expression of dairy goat ovary miRNAs was analyzed by Solexa/Illumina high-throughput sequencing technology,which producing same-sex lambs,thus providing a basis for exploring the role of miRNAs in the regulation of fertility.The total RNA was extracted from the ovaries of the dairy goat mothers with same-sex-lambs.The eDNA library was established and cloned,sequenced,and conducted bioinformatics analysis.The results show there were 744,782 miRNAs detect…查看全部>>
李广;张效川;赵海波;高特仰
西北农林科技大学动物科技学院,陕西杨凌712100西北农林科技大学动物科技学院,陕西杨凌712100西北农林科技大学动物科技学院,陕西杨凌712100西北农林科技大学动物科技学院,陕西杨凌712100
农业科技
奶山羊卵巢miRNAs
dairy goatovarymiRNAs
《家畜生态学报》 2017 (11)
21-26,6
陕西省自然科学基础研究计划项目(2015JM3087)
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