家畜生态学报2017，Vol.38Issue(11)：53-57,5.
紫花苜蓿HSC70-2基因克隆及其时空表达
Cloning and Spatio-temporal Expression of HSC70-2 Gene from Medicago sativa
摘要
Abstract
In this study,a MsHSC70-2 gene was determined by polymerase chain reaction (PCR),and its mRNA sequence is 2025bp,which contains a complete open reading box of 1950bp,encoding 649 amino acids,molecular weight of the protein is about 70.97 Kda,its isoelectric point is about 4.74.The mRNA content of MsHSC70-2 from different organs of the variety in different seasons were detected by qRT-PCR,showing that its content in the same organs had significant difference in different seasons(P＜0.05),and the content of MsHSC70-2 mRNA among organs was different during summer(P＜0.01).The expression trend of the gene in root,stem,leaf and bud increased first and then decreased from April to September,demonstrating that the expression of MsHSC70-2 is regulated by day length and temperature,and its expression variation affects the growth and development of various organs,its high expression promotes the growth and development of alfalfa,and plant growth was inhibited at low expression level,showing that decrease of its expression may participate in fall dormancy in alfalfa.The study provides significant basis for further study on the function of alfalfa HSC70-2.关键词
MsHSC70-2/苜蓿/耐热性/时空表达Key words
MsHSC70-2/Alfalfa/heat resistance/spatio-temporal expression分类
农业科技引用本文复制引用
杜红旗,姜山,史莹华,王成章..紫花苜蓿HSC70-2基因克隆及其时空表达[J].家畜生态学报,2017,38(11):53-57,5.基金项目
农业部公益性行业科研专项项目(No.201403048-6) （No.201403048-6）
国家牧草产业技术体系(No.CARS-35) （No.CARS-35）
国家星火计划项目(No.2015GA750003) （No.2015GA750003）
