家畜生态学报2017，Vol.38Issue(11)：62-67,6.
牦牛源巴氏杆菌对氟喹诺酮类药物的耐药表型及耐药基因检测
Detection of Resistant Phenotype and Resistance Genes to Fluoroquinolones among Pasteurella Strains Isolated from Yaks
摘要
Abstract
220 samples of yak lung tissues were collected from large-scale farms and beast prevention stations (bureaus) in different regions of Tibet,and 44 strains of Pasteurella were isolated through microscopic examination of the bacteria culture characteristics,dyeing,microbial identification system.The broth microdilution method was adopted to determine the antibacterial activity of fluoroquinolones and colistin against 44 strains of bacteria.Specific primers were designed,and synthesis and resistance genes were detected by PCR.The results showed that minimum inhibitory concentration (MIC) range of six fluoroquinolone drugs was between 0.125-128μg/mL,the MIC of colistin in the range of ＜0.016-8μg/mL.Most of the pasteurella strains showed levels of resistant rate to ciprofloxacin (52.27％),and gatifloxacin (54.55％),but low resistance was found forcolistin,with 4.55％,the MIC of colistin as low as below ＜2 μg/mL.The results revealed that the fluoroquinolones resistance genes,qnrA,qnrB,qnrS,and aac(6')-Ib-cr were detected and the positive rates among these strains were 29.55％,18.18％,22.73％ and 18.18％,respectively,but qnrC,qnrD,qepA genes were not checked out.This research shows that most of the strains against fluoroquinolone drugs were cross-resistant,therefore the monitoring of drug resistance and resistance gene should be enhanced clinically.关键词
牦牛/巴氏杆菌/氟喹诺酮类/耐药表型/耐药基因Key words
yak/pasteurella/fluoroquinolones/resistant phenotype/drug resistance genes分类
农业科技引用本文复制引用
宋博利,蔡田,刘保光,孙华润,李帆,丁海峰,李小申,吴华..牦牛源巴氏杆菌对氟喹诺酮类药物的耐药表型及耐药基因检测[J].家畜生态学报,2017,38(11):62-67,6.基金项目
西藏自治区高校青年教师创新支持计划项目(QCZ2016-88和QC2015-77) （QCZ2016-88和QC2015-77）
西藏自治区自然科学基金项目(2016ZR-15-62和2016ZR-ZX-01) （2016ZR-15-62和2016ZR-ZX-01）
