军事医学2017，Vol.41Issue(8)：652-657,6.DOI:10.7644/j.issn.1674-9960.2017.08.007
二甲双胍对PC12细胞低氧损伤的保护作用
Protective effect of metformin on PC12 cell injury induced by hypoxia
摘要
Abstract
Objective To investigate the effect of metformin on rat adrenal pheochromocytoma PC12 cell injury induced by hypoxia and its possible molecular mechanisms.Methods PC12 Cells were cultured under hypoxia conditions (0.3％ O2).The cell survival and apoptosis were detected by methylrosanilinium chloride (crystal violet) staining and Annexin-V/propidium iodide (PI) staining,the cell viability measured by CCK8 assay,and the signaling activity and protein levels were detected by Western blotting.Results Physical hypoxia at an O2 concentration of 0.3％ resulted in time-dependent injury to PC12 cells,while metformin pretreatment had protective effect on PC12 cell injury induced by hypoxia.AMP-activated protein kinase (AMPK) signaling was activated by metformin treatment,and AMPK kinase inhibitor compound C blocked the protective effect of metformin on hypoxia-induced PC12 cell injury.Conclusion These results demonstrate that metformin pretreatment has protective effect on PC12 cell injury induced by hypoxia,and AMPK signaling plays an important role in the protective effect of metformin.关键词
二甲双胍/低氧损伤/腺苷酸活化蛋白激酶/PC12细胞Key words
metformin/hypoxia injury/AMP-activated protein kinase/PC12 cells分类
医药卫生引用本文复制引用
林霄,成祥,黄欣,赵彤,范明,张毅,赵名,朱玲玲..二甲双胍对PC12细胞低氧损伤的保护作用[J].军事医学,2017,41(8):652-657,6.基金项目
国家自然科学基金重点资助项目(81430044) （81430044）
北京市科技计划资助项目(Z161100000216314) （Z161100000216314）
