军事医学2017，Vol.41Issue(8)：689-693,5.DOI:10.7644/j.issn.1674-9960.2017.08.015
基于时间分辨荧光纳米颗粒的磁分离均相免疫分析方法
Development of time resolved fluorescence microparticle-conjugated anti-human procalcitonin antibody for homogeneous immunoassay
吴灿军 1律清宇 2郝淮杰 2赵成娜 2郑玉玲 1姜永强2
作者信息
- 1. 安徽医科大学,合肥230032
- 2. 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071
- 折叠
摘要
Abstract
Objective To develop europium (Ⅲ) [Eu (Ⅲ)] chelated microparticles for homogeneous immunoassay.Methods Anti-human PCT antibodies were labeled with Eu (Ⅲ) chelated nanoscale microparticles as the detection antibody,and another anti-human PCT antibody was labeled with biotin as the solid-phase antibody.Magnetic microspheres labeled with streptavidin were used to separate the complexes of Eu-IgM-PCT-IgM-Biotin.Results In the homogeneous immunoassay,the standard curve fit was not linear.The quadratic curve was Y =19170.12 + 75493.74X-26.00X2(r =0.9986).According to the standard curve,the limit of detection for PCT was 0.04 ng/ml.Conclusion The homogeneous immunoassay which uses Eu (Ⅲ) chelated microparticles is highly sensitive for detection of PCT recombinant antigens and may serve as a promising method to measure serum PCT levels in the future.关键词
降钙素原/时间分辨荧光法/铕离子荧光微球/磁珠/均相检测Key words
procalcitonin/time-resolved fluoroimmunoassay/europium (Ⅲ) chelated microspheres/magnetic microspheres/homogeneous immunoassay分类
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吴灿军,律清宇,郝淮杰,赵成娜,郑玉玲,姜永强..基于时间分辨荧光纳米颗粒的磁分离均相免疫分析方法[J].军事医学,2017,41(8):689-693,5.
