临床口腔医学杂志2017，Vol.33Issue(12)：707-711,5.DOI:10.3969/j.issn.1003-1634.2017.12.002
过表达Fpr2对树突状细胞成熟和趋化功能的调控作用
The regulatory effect of over-expression of Formyl Peptide Receptor-2 on dendritic cell maturation and chemo-taxis
摘要
Abstract
Objective:To investigate the regulatory effect of over-expression of Formyl Peptide Receptor-2(Fpr2)on dendritc cell maturation and chemotaxis. Methods:Dendritic cell line DC2. 4 was transfected with plasmid DNA pEGFP-Fpr2 to upregulate the expression of Fpr2 with the aid of a targeted peptide RVG-P. The concentration of selected cytokines(IL-6, IL-10,TNF-α,IL-12 p70)was measured by using the ELISA KIT. The expression levels of surface marker molecules(CD40, CD80,CD83,CD86,MHCII) were measured by FACS. Migration assay was performed by using Transwell migration cham-bers. Western blot was performed to evaluate the phosphorylated forms of intracellular signal transducers of MAPK ( P38, ERK1/2,JNK). Results:The expression levels of CD83 in DC2. 4 increased upon up-regulation of Fpr2. The cytokine levels of IL-6,IL-10 and TNF-α increased after up-regulation of Fpr2. The average number of cell migration decreased after plas-mid DNA transfection. However,the migration ability of pEGFP-Fpr2 transfected cells increased compared with the pEGFP-N1 transfected cells. Phosphorylation levels of P38,JNK and ERK1/2 in the pEGFP-Fpr2 transfected cells were observed with a significant increase when compared with the pEGFP-N1 transfected cells. Conclusion:Over-expression of Formyl Pep-tide Receptor-2 promoted maturation and chemotaxis of dendritic cells.关键词
树突状细胞/甲酰肽受体/成熟/趋化Key words
Dendritic cells/Formyl peptide receptor/Maturation/Chemotaxis分类
生物科学引用本文复制引用
张舟,许庆安..过表达Fpr2对树突状细胞成熟和趋化功能的调控作用[J].临床口腔医学杂志,2017,33(12):707-711,5.基金项目
国家自然科学基金资助项目(81570968) （81570968）
