摘要
Abstract
Objective:The purpose of this study was to investigate the expression of DKK1,SFRP1,Runx2 and Os-teocalcin in experimental periapical lesions in mice. Methods:Periapical lesions were induced in 50 C57 BL/6 mice by oc-clusal pulp exposure in the mandibular first molars of mice. Both sides of mandibular first molars were exposed. Five groups of animals receiving pulp exposure were sacrificed at 7,14,21 and 28 days(n=7,each group),and nontreated mice served as the baseline control(day 0). Animals were sacrificed randomly at 0,7,14,21 and 28 days after pulp exposure. The jaws that contain the first molar were obtained and subject to Micro-CT,histological,immunohistochemical examination. At the 14th day before sacrified,the mice(n=3,each group)were injected calcein(30 mg/kg),and the 4th days prior to sacrifice,the mice were injected fluorochromes alizarin(50 mg/kg),for assessment of bone formation activity. Results:According to the Micro-CT examination,the periapical shadow could beobserved on day 7,and increased to peak on day 21. The number of osteoclasts ascended and peaked on day 21,and then gradually decreased on day 28. DKK1 and SFRP1 positive cells could be observed ascended from day 7 to day 21,and then decreased gradually on day 28. The number of Runx2 and Osteocalcin positive cell increased from day 7 to day 28. Conclusion:DKK1,SFRP1 might be involved in periapical lesions. It may be a complementa-ry therapeutic strategy for bone loss in mice periapical.关键词
DKK1/SFRP1/Runx2/Osteocalcin/根尖周炎Key words
DKK1/SFRP1/Runx2/Osteocalcin/Periapical lesions分类
医药卫生
