临床口腔医学杂志2017，Vol.33Issue(12)：719-722,4.DOI:10.3969/j.issn.1003-1634.2017.12.004
microRNA-146a在口腔鳞状细胞癌中的表达及临床意义
The expression and clinical significance of microRNA-146a in oral squamous cell carcinoma
窦尉尉 1岳从雷 1刘洪伟 2徐宁 1吕庆 1高德3
作者信息
- 1. 济宁口腔医院口腔颌面外科 山东 济宁 272000
- 2. 济宁市第一人民医院口腔颌面外科 山东 济宁 272000
- 3. 济宁口腔医院牙体牙髓科 山东 济宁 272000
- 折叠
摘要
Abstract
Objective:To evaluate the expression of microRNA-146a in oral squamous cell carcinoma(OSCC)tis-sues and cell lines,and to assess the effect of microRNA-146a on migration and invasion in OSCC cells. Methods:Quantita-tive real-time PCR(qRT-PCR)was used to assess the expression levels of microRNA-146a in 60 cases of OSCC tissues as well as 30 cases of normal oral mucosa tissues,followed by analyses on the correlation between microRNA-146a expression and the clinicopathologic parameters of OSCCs. Meanwhile,the expression levels of microRNA-146a in human OSCC cell lines(SCC4,SCC15,Tca8113)andnormalhuman oral keratinocyte line(HOK)were detected by qRT-PCR. In addition,the migration and invasion abilities of OSCC cells after up-regulating microRNA-146a were assessed by Transwell migration and invasion assays. Results:The microRNA-146a expression was significantly lower in OSCC tissues compared to that in normal oral mucosa tissues(P<0. 05),and the microRNA-146a expression was significantly associated with lymph node metastasis and clinical stage(P<0. 05). Moreover,the microRNA-146a expression was significantly lower in OSCC cell lines than that in normal oral keratinocyte line(P<0. 05). Transwell migration and invasion assays demonstrated that migration and invasion abilities of Tca8113 cell was significantly suppressed by up-regulating microRNA-146a expression(P<0. 05). Conclusion:The expression of microRNA-146a was decreased in OSCC tissues and cells. The migration and invasion abilities of OSCC cells were significantly inhibited by up-regulating microRNA-146a,which was a potential targetingene therapy of OSCC.关键词
口腔鳞状细胞癌/microRNA-146a/迁移/侵袭Key words
Oral squamous cell carcinoma/MicroRNA-146a/Migration/Invasion分类
生物科学引用本文复制引用
窦尉尉,岳从雷,刘洪伟,徐宁,吕庆,高德..microRNA-146a在口腔鳞状细胞癌中的表达及临床意义[J].临床口腔医学杂志,2017,33(12):719-722,4.
