农业环境科学学报2017，Vol.36Issue(10)：1960-1967,8.DOI:10.11654/jaes.2017-0430
镉对“标准化”背角无齿蚌的急性毒性及脂质过氧化和DNA损伤的影响
Acute toxicity of cadmium and its effects on lipid peroxidation and DNA damage in "standardized" Anodonta woodiana
摘要
Abstract
This study was undertaken to investigate the toxic effect of waterborne Cd2+ on the freshwater bivalve "standardized" Anodonta woodiana,a unique bioindicator used in the "Freshwater Mussel Watch" project.Glochidia(＜ 24 h,mean length 0.25 mm),early juveniles (＜ 5 days,mean length 0.27 mm),and late juveniles(6 months,mean length 50 mm) were exposed to various Cd2+ concentrations for the acute toxicity test.Furthermore,late juveniles or adults (48 months,mean length 85 mm) were used to investigate the temporal variation of lipid peroxidation[malondialdehyde(MDA) content] and DNA damage[Olive tail moment(OTM)] in gills,which are the target organs of Cd2+ accumulation.The results showed that the 24-h median effect concentration(EC50) for glochidia was 0.001 8 mg· L-1,whereas the 96-h EC50 values for early and late juveniles were 0.004 7 mg ·L-1 and 4.5 mg ·L-1,respectively.MDA content was continuously reduced over time in both the 0.05 mg· L-1 and 0.5 mg· L-1 groups,and was not related to Cd2+ concentration(P＞0.05).In contrast,the OTM values increased over time,and were linearly and positively correlated with Cd2+ concentration(P＜0.05).These results indicate that glochidia and early juveniles may be used as sensitive test organisms for Cd2+ toxicology research,late juveniles and adults may be suitable for biomonitoring Cd2+ pollution,and OTM is a valuable biomarker for early warning of Cd2+ contamination.关键词
“标准化”背角无齿蚌/生活史阶段/镉/急性毒性/生物标志物Key words
"standardized" Anodonta woodiana/life stages/cadmium/acute toxicity/biomarker分类
资源环境引用本文复制引用
陈修报,刘洪波,苏彦平,姜涛,杨健..镉对“标准化”背角无齿蚌的急性毒性及脂质过氧化和DNA损伤的影响[J].农业环境科学学报,2017,36(10):1960-1967,8.基金项目
国家自然科学基金青年科学基金项目(31502166) （31502166）
江苏省自然科学基金项目(BK20161144) （BK20161144）
中央级公益性科研院所基本科研业务费专项资金项目(2015JBFM13)The Young Scientists Fund of the National Natural Science Foundation of China(31502166) （2015JBFM13）
The Natural Science Foundation of Jiangsu Province,China(BK20161144) （BK20161144）
Central Public-interest Scientific Institution Basal Research Fund(2015JBFM13) （2015JBFM13）
