农业环境科学学报2017，Vol.36Issue(10)：2039-2047,9.DOI:10.11654/jaes.2017-0358
施用猪粪土壤中抗生素的降解、植物有效性及土壤酶活性变化
Degradation and phytoavailability of antibiotics in soils amended with pig manure and changes of soil enzyme activity
摘要
Abstract
A soil incubation and a pot culture experiment using litchi plants(Litchi chinensis Sonn.) were conducted using soils amended with various rates(0,1％,2％,and 4％,W/W) of pig manure from an intensive animal farm,to investigate degradation of antibiotics(chlortetracycline and doxycycline) and changes of soil enzyme activity within 360 d,and examine uptake of antibiotics from pig manure by litchi plants at different harvest times(153 d and 210 d).The concentrations of both antibiotics in soils were significantly increased by application of pig manure(P＜0.05).The half-lives of chlortetracycline and doxycycline were 26.84 d and 58.89 d,respectively.Application of pig manure enhanced the activities of soil catalase,urease,and acid phosphatase.Moreover,the activities of the three enzymes were positively correlated to the concentrations of soil chlortetracycline and doxycycline when pig manure was applied at aa rate of 1％ or 2％ (P＜0.05).Litchi plants absorbed both antibiotics.With prolonged growth time,chlortetracycline concentration in plant significantly increased,but was not related to the level of pig manure used.The doxycycline concentration in the plants significantly increased as the manure level increased,but was diluted by the litchi biomass.Chlortetracycline was easily translocated upward,with the transfer factor of 0.595～0.789;however,translocation of doxycycline was limited,with a transfer factor less than 0.01.After the harvest of litchi plants,the concentration of both antibiotics in rhizosphere soil was significantly increased by an increased rate of manure application.Because litchi is a perennial woody fruit tree,successive and extensive use of animal manure would result in accumulation of antibiotics from the manure in the soil,potentially leading to absorption of antibiotics,particularly chlortetracycline,by the edible litchi fruit.关键词
粪肥/抗生素/土壤/荔枝/植物有效性Key words
animal manure/antibiotics/soil/litchi/phytoavailability分类
资源环境引用本文复制引用
石慧,白翠华,周昌敏,罗东林,姚丽贤..施用猪粪土壤中抗生素的降解、植物有效性及土壤酶活性变化[J].农业环境科学学报,2017,36(10):2039-2047,9.基金项目
国家现代农业产业技术体系建设专项(CARS 33-08) （CARS 33-08）
广东省省级科技计划项目(2014A020208095)The Earmarked Fund for Modern Ago-industry Technology Research System(CARS 33-08) （2014A020208095）
Science and Technology Planning Project of Guangdong Province(2014A020208095) （2014A020208095）
