农业环境科学学报2017，Vol.36Issue(10)：2114-2120,7.DOI:10.11654/jaes.2017-0490
日粮添加地衣芽孢杆菌对仔猪发酵床垫料理化性质及微生物群落的影响
Effects of diet with Bacillus licheniformis on physicochemical properties and microbial community in litters of piglet biobed
摘要
Abstract
To investigate the effects of Bacillus licheniformis on the physicochemical properties and microbial community in litters of piglet biobed,weaned piglets were fed in three groups.The first group was fed a basal diet plus B.licheniformis (probiotics treatment,PBT),the second group was fed a basal diet plus bacitracin zinc and sulfate prime enemy(antibiotic treatment,ABT),and the last group was fed a basal diet only (control treatment,CT).The results showed that the activity of proteinase in PBT was significantly improved (P＜0.05),while no significant difference in urease activity or ammonium concentration was observed among the three treatments(P＞0.05).On day 15,the total number of cells of Actinomyces in ABT was significantly increased(P＜0.05).On day 35,the number of Bacillus in PBT was significantly increased (P＜0.05).On day 49,the number of Bacillus in PBT was significantly higher than that in ABT (P＜0.05),and the number of Staphylococci in ABT was significantly lower than that in CT(P＜0.05).The numbers of Escherichia coli in ABT and PBT were lower than that in CT.The total numbers of bacterial and Bacillus in PBT were lower than that in CT,although these differences were not significant(P＞0.05).The results indicated that dietary antibiotics might inhibit the growth of pathogenic microorganisms,while the probiotic bacteria population might also be affected.No significant difference(P＞0.05) was observed in the DGGE Shannon-Wiener index or the evenness between the three treatments.In general,the addition of B.licheniformis significantly increased the population of Bacillus.The protease activity in the litters of piglet biobed and the degradation efficiency of the manure were improved,while no significant change was observed in the bacterial community diversity.关键词
地衣芽孢杆菌/垫料/理化性质/微生物群落Key words
Bacillus licheniformis/biobed litters/physicochemical properties/microbial community分类
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宦海琳,白建勇,闫俊书,周维仁,徐小明,顾洪如..日粮添加地衣芽孢杆菌对仔猪发酵床垫料理化性质及微生物群落的影响[J].农业环境科学学报,2017,36(10):2114-2120,7.基金项目
江苏省农业自主创新资金项[CX13(5040)] （5040）
江苏省农业三新工程项目(SXGC[2016]331)Jiangsu Agriculture Science and Technology Innovation Fund[CX (13)5040] （SXGC[2016]331）
Jiangsu agriculture Province New Project Fund (SXGC[2016]331) （SXGC[2016]331）
