山东科学2017，Vol.30Issue(6)：22-28,7.DOI:10.3976/j.issn.1002-4026.2017.06.004
正交试验法优选黄芩配方颗粒的水提工艺
Optimization of the water extraction conditions of Huangqin dispensing granules by orthogonal test
摘要
Abstract
For the optimization of the water extraction conditions of Huangqin dispensing granules,orthogonal test method was used to investigate the effects of 3 factors,including different water volumes,extraction frequency and extraction duration,on the total content of baicalin,wogonoside,baicalein and wogonin and the extract rate.Meanwhile,the methodology of the HPLC determination was verified.As a result,under the experiment conditions,the importance order was as follows:extraction duration,extraction frequency,water volume.And extraction durations at different level were significant different (P＜0.05).The experiment proved that the suitable extraction condition was that Huangqin slices were added 10 times volume of water and extracted 3 times for 1 h each time.The extraction condition optimized in this paper was practical and stable with high extraction rate of active ingredients,which was suitable to be used in the preparation of Huangqin dispensing granules.关键词
正交试验/黄芩配方颗粒/水提工艺Key words
orthogonal test/Huangqin dispensing granules/water extraction conditions分类
医药卫生引用本文复制引用
郝翠,穆岩,赵恒强,崔莉,刘伟,刘逢芹..正交试验法优选黄芩配方颗粒的水提工艺[J].山东科学,2017,30(6):22-28,7.基金项目
山东省中医药科技发展计划(2013-201) （2013-201）
山东省科技发展计划(2013GSF1120) （2013GSF1120）
山东省自然科学基金(ZR2013HM075,ZR2016YL006) （ZR2013HM075,ZR2016YL006）
