山东科学2017，Vol.30Issue(6)：29-34,6.DOI:10.3976/j.issn.1002-4026.2017.06.005
利用斑马鱼模型研究芦笋有效成分对免疫功能的调节作用
The immune-regulatory effects of active ingredients of asparagus using zebrafish model
摘要
Abstract
In this experiment,48 hpf (hour post fertilization) healthy transgenic zebrafish Tg(lyz:EGFP) was used as animal model.Firstly,immune suppression of zebrafish embryos was induced by chloramphenicol treatment.Then those zebrafish embryos were treated with 25.0～ 200.0 mg/L asparagus polysaccharide and 6.25 ～ 100.00 mg/L asparagus saponins for 24 h,respectively.Finally,the development of zebrafish in each group was observed under the microscope,and the number of neutrophils in zebrafish was recorded and counted to evaluate their immune-regulatory effects.The results showed that the numbers of neutrophils in 72hpf zebrafish trunk area decreased in chloramphenicol-induced group.Asparagus polysaccharide group(50.0～200.0 mg/L) showed a significant immunological enhancement effect with dosedependent manner.Asparagus saponins could also significantly improve chloramphenicol-induced immunosuppressive effects within the experimental dose range.In this study,This study showed that the active ingredients of asparagus,such as polysaccharide and asparagus saponin,had obvious regulation effects on the immune function of zebrafish,which provided the scientific basis for further development of asparagus.关键词
芦笋/芦笋多糖/芦笋皂苷/免疫调节/斑马鱼Key words
Asparagus/Asparagus polysaccharide/Asparagus saponins/immune-regulation/zebrafish分类
医药卫生引用本文复制引用
刘发生,刘可春,王荣春,郭敬兰,陈艳莉,夏青,孔浩天,冯润良,韩利文,张爱平..利用斑马鱼模型研究芦笋有效成分对免疫功能的调节作用[J].山东科学,2017,30(6):29-34,6.基金项目
山东省重点研发计划(2016GSF121009,2016GGC01089) （2016GSF121009,2016GGC01089）
山东省自然科学基金(ZR2016YL009) （ZR2016YL009）
国家海洋公益性行业科研专项(201505030) （201505030）
