山东农业大学学报（自然科学版）2017，Vol.48Issue(6)：801-806,6.DOI:10.3969/j.issn.1000-2324.2017.06.001
不同发育时期鸡下丘脑和卵巢组织中内参基因表达稳定性分析
Stability Analysis of Reference Genes Expression in Chicken Hypothalamus and Ovary Tissues during Different Developmental Stages
摘要
Abstract
In this paper, the expression stability of the three common house-keeping genes in the hypothalamus and ovary of chicken at different stages of development was analyzed, this provided the scientific basis for the selection of quantitative detection of related functional genes on gonadal axis of chickens. Jining Bairi chicken at different sexual development stages as experimental material, used the real-time fluorescence quantitative PCR detecting common housekeeping gene actin gene beta-(beta-actin), 3-glyceraldehyde-3-phosphate dehydrogenase gene (glyceraldehyde-3-phosphate dehydrogenase GAPDH) and 18S ribosomal RNA gene (18S rRNA) expression in hypothalamus and ovarian tissue. The softwares of geNorm, NormFinder and BestKeeper were used to compare the stability of these genes expression. The results showed at the different stages of sexual development of Jining Bairi chicken, GAPDH gene was the most stable expression in the hypothalamus, and the most stable expression in the ovaries was the beta actin gene. The results provide a reference for the accurate analysis of the relative expression of genes on the HPG of chicken by real-time fluorescence quantitative PCR.关键词
持家基因/实时荧光定量PCR/鸡/卵巢/下丘脑Key words
Housekeeping gene/qRT-PCR/ovary/hypothalamus分类
农业科技引用本文复制引用
袁振杰,陈秋月,姜运良,唐辉,李显耀,康丽..不同发育时期鸡下丘脑和卵巢组织中内参基因表达稳定性分析[J].山东农业大学学报（自然科学版）,2017,48(6):801-806,6.基金项目
山东省自然科学基金(ZR2013CM014) （ZR2013CM014）
国家自然科学基金(31301962) （31301962）
山东省高等学校科技计划(J12LF02) （J12LF02）
山东省农业生物资源创新利用研究(家禽种质创新利用研究) （家禽种质创新利用研究）
山东省现代农业产业技术体系项目(SDAI T-13-011-02) （SDAI T-13-011-02）
