山东农业大学学报（自然科学版）2017，Vol.48Issue(6)：832-836,5.DOI:10.3969/j.issn.1000-2324.2017.06.006
氘代同位素内标液相色谱-质谱联用测定黄酒中的氨基甲酸乙酯
Determination of Ethyl Carbamate in Chinese Rice Wine by HPLC/MS-MS Using Deuterium Isotope as Internal Standard
摘要
Abstract
A high performance liquid chromatography with mass spectrometric detection method was established for the direct determination of ethyl carbamate in Chinese rice wine. The samples were diluted with methanol-water (v:v=25:75), filtered through 0.22μm microporous membrane. The LC separation was performed on a Waters HPLC system with a SunFireTM C18 column, methanol and 0.1％(v/v)acetic acid aqueous solution as the mobile phase. The ethyl carbamate was determined in the mode of electrospray positive ionization (ESI+) and multiple reaction monitoring (MRM). The D5-EC was used as the internal standard for the quantitative analysis . Carbamate ethyl ester had good relationship between 2～500 mu g/L and the correlation coefficient was 0.9922,the detection limit of ethyl carbamate in yellow rice wine was 1.8mu g/L, and the quantitative limit was 5.0 mu g/L, and the recovery rate was 88.9％～103.1％. The RSD of relative standard deviation was 1.8％～5.6％, this method is easy, fast, accurate and suitable for the determination of ethyl carbamate in Chinese rice wine.关键词
高效液相色谱/串联质谱/氨基甲酸乙酯/黄酒Key words
High performance liquid chromatography (HPLC)/mass spectrometry/Ethyl carbamate/Chinese rice wine分类
轻工纺织引用本文复制引用
贾素中,欧杰,王婧,许禄..氘代同位素内标液相色谱-质谱联用测定黄酒中的氨基甲酸乙酯[J].山东农业大学学报（自然科学版）,2017,48(6):832-836,5.基金项目
上海市科委工程中心能力提升项目(16DZ2280300) （16DZ2280300）
