山东农业大学学报（自然科学版）2017，Vol.48Issue(6)：853-857,5.DOI:10.3969/j.issn.1000-2324.2017.06.010
不同磁珠对过敏原蛋白纯化效果的比较研究
Comparison of Different Magnetic Beads for Purification of Allergen Protein
摘要
Abstract
In this study, the main allergen tropomyosin (Tm) of Penaeus vannamei was used as the purification object. Two commercial magnetic nanoparticles were selected to build immunomagnetic beads. The allergen was purified by immunomagnetic separation technology. SDS-PAGE, western blot and protein quantification methods were employed to identify the purified sample , and the effect of different immune magnetic beads for the purification of allergen was compared. Results showed that antibody coupling amount of magnetic bead A was higher than that of magnetic beads B. Besides, the combination and elution rates of immune magnetic beads A for antigen was 1.5 and 1.8 times than that of immune magnetic bead B, respectively;the capture rate of allergen by immunomagnetic beads A was 47.9％, while immunomagnetic beads B was only 17.6％. By comparing the physicochemical and structural properties of the two beads, the magnetic bead with larger bead-size and poor dispersion was gathered during the coupling process, which reduced the antibody coupling efficiency and affect the purification effect. This paper showed that magnetic bead with good dispersion and high antibody coupling rate is more suitable for the separation and purification of protein. This study provides a theoretical basis and experimental basis for the purification of allergens in the future.关键词
磁珠/过敏原纯化/原肌球蛋白/免疫磁分离技术Key words
Magnetic bead/allergen purification/tropomyosin/immunomagnetic separation分类
化学化工引用本文复制引用
李文娇,阎俊,杜娟,黄天娇,卢瑛..不同磁珠对过敏原蛋白纯化效果的比较研究[J].山东农业大学学报（自然科学版）,2017,48(6):853-857,5.基金项目
上海市农委科技兴农重点攻关项目(沪农科攻字(2016)第4-4号) （沪农科攻字(2016）
上海市科委地方院校能力建设项目(15320502100) （15320502100）
上海市科委工程中心能力提升项目(16DZ2280300) （16DZ2280300）
