陕西中医2018，Vol.39Issue(1)：3-5,3.DOI:10.3969/j.issn.1000-7369.2018.01.00
柴胡皂苷d-黄芩苷配伍对CCl4诱导大鼠HSC的TLR4-NF-κB通路作用研究
Inhibitory effects of SaikosaponinD-Baicalin on activation of rat HSC induced by CCl4
摘要
Abstract
Objective:To investigate the inhibitory effect and mechanism of SaikosaponinD-Baicalin on activa-tion of rat HSC induced by CCl4.Methods:The cells were divided into the following groups:control group ( no treat-ment),model group ( treated with 6mmol/L CCl4 treated for 24 h),SB groups (pretreated with 6mmol/L CCl4 trea-ted for 24 h and2.5,5,10μg/mlSB for 24 h ).Concentrations of HA,LN,PCIII,IV-C were assayed by ELISA kits.Gene,protein expression of TLR4 and NF-κB were examined by RT-PCR and western blot analysis.Results:SB,TAK-242 and BYA 11-7082 could significantly inhibit cell proliferation induced by CCl4.Furthermore they can downregulated gene and protein expressions of NF-κB and the secretion of HA,LN,PCIII,IV-C ( P<0.001).Fur-ther studies disclosed that the SB and TAK-242 could significantly inhibit the gene and protein expressions of TLR4.Con-clusion:SB could inhibit inflammatory responses in HSC induced by CCL 4 probably by inhibiting the transcription activity and protein expression of TLR4-NF-κB which indicates its possible therapeutic effect in liver fibrosis.关键词
肝纤维化/柴胡皂苷D-黄芩苷/肝星形细胞/CCL4诱导/TLR4/NF-κBKey words
Liver fibrosis/Saikosaponin/D-Baicalin/HSC/Induced by CCl4/TLR4/NF-κB分类
医药卫生引用本文复制引用
李敏,高凯,王华林,王義雯,李静,王斌..柴胡皂苷d-黄芩苷配伍对CCl4诱导大鼠HSC的TLR4-NF-κB通路作用研究[J].陕西中医,2018,39(1):3-5,3.基金项目
陕西省科技厅项目(2013JK4023)陕西省中医药管理局项目(ZY06)陕西省教育厅项目(12JK1016) （2013JK4023）
