陕西中医2018，Vol.39Issue(1)：10-13,4.DOI:10.3969/j.issn.1000-7369.2018.01.003
参芪止疱颗粒抗HSV-2的体外实验研究
Experimental research on effect of Shenqi Zhipao granule against the herpes simplex viruses type 2 in vitro
摘要
Abstract
Objective:To explore the effect of Shenqi Zhipao granule in inhibiting the herpes simplex viruses type 2 (HSV-2) in vitro.Methods:HFF was infected with HSV-2 and was made an intervention with Shenqi Zhipao granule after infection,at the same time,the acyclovir was used to make a comparison.Methods of CPE and MTT colorimetric assay were used to conduct this experiment.Results:The results showed that the TCID50 of HSV-2 was 10-4,the CC50 of Shenqi zhipao granule in HFF was more than 10mg/ml,indicating that Shenqi Zhipao granule had less toxicity for HFF,in the experiment of drugs inhibiting viral replication,the EC50 of Shenqi Zhipao granule was 0.30 mg/ml in infected cells,TI>36,indicating that Shenqi zhipao granule had a protection for HFF infected with HSV-2,In the therapeutic experiment of HFF infected with HSV-2,the EC50 of Shenqi Zhipao granule was 0.60mg/ml,TI>25,indicating that Shenqi Zhipao Granule had an inhibitory action for viral replication after infection and had no effect in preventing HSV-2,the phases of it in inhibiting HSV-2 replication was mainly at 0 h,2 h,4 h,besides,Shenqi Zhipao Granule had no effect in inhibiting the attachment and invasion of HSV-2 on cells.Conclu-sion:Shenqi Zhipao Granule has the effect of antivirus in vitro,it deserves further clinical popularization.关键词
生殖器疱疹/@参芪止疱颗粒/@人包皮成纤维细胞/@2-型单纯疱疹病毒Key words
Genital herpes/@Shenqi Zhipao granule/@Human foreskin fibroblast/@Herpes simplex vi-ruses type 2分类
医药卫生引用本文复制引用
候亚林,杜立行,欧柏生,孙亚如,黄涛,黄彦,王艺..参芪止疱颗粒抗HSV-2的体外实验研究[J].陕西中医,2018,39(1):10-13,4.基金项目
国家自然科学基金资助项目(81160442) （81160442）
