生物技术通报2017，Vol.33Issue(12)：61-66,6.DOI:10.13560/j.cnki.biotech.bull.1985.2017-0599
鱼类及两栖动物抗菌肽的研究进展
Research Advances on Antimicrobial Peptides of Fish and Amphibians
摘要
Abstract
At present,750 antimicrobial peptides have been successfully isolated from the biological community,of which with antitumor activity account for about 13.1%. The antibacterial peptides derived from amphibians and fish are about 13.3% and 9.3%, respectively. Fish and amphibians are the most primitive vertebrate,and most of them rely on the gills,skin and intestinal breathing. Due to their extremely fragile acquired immune system,they have a powerful innate immune system to avoid the infection of the pathogen,thus the antimicrobial peptides distributed in different parts have become the important component of first defense line of broad-spectrum in these lower organisms. This paper reviewed the broad-spectrum antibacterial activity and immune regulation ability of antibacterial peptides such as moronecidin,epinecidin-1,paradaxin,and misgurin distributed in hybrid striped bass,Epinephelus coioides,Pardachirus marmoratus, and Misgurnus anguillicaudatusis,as well as antibacterial spectrum of smelly frog antimicrobial peptide. For instance,the moronecidin can kill all gram positive bacteria and some gram negative bacteria. Epinecidin-1 can effectively prevent the infection of vulnificus(Vibrio vulnificus) in grouper and kill the nervous necrosis virus. Paradaxin reveals the antitumor activities. Misgurin has strong bactericidal ability. Antibacterial peptides from the smelly frog can kill Candida albicans. Then,the paper summarized the antibacterial peptides successfully expressed in Pichia pastoris and those derived from fish and amphibian of having antiviral and antitumor activities. Finally,the paper discussed the problems and solutions in the application of these antibacterial peptides,providing a theoretical basis for green and effective antibacterial peptide drugs in various fields to play a great role.关键词
抗菌肽/抗菌谱/毕赤酵母Key words
antimicrobial peptides/antimicrobial spectrum/Pichia pastoris引用本文复制引用
薛林贵,马萍,尚海,何小燕,陈熙明,刘光绣,陈拓,张威..鱼类及两栖动物抗菌肽的研究进展[J].生物技术通报,2017,33(12):61-66,6.基金项目
国家自然科学基金项目(31260135,31400437),甘肃省科技支撑计划项目(1504FKCA015),兰州市科技计划项目(2015-3-68) （31260135,31400437）
