摘要
Abstract
Objective To establish a rapid screening method for the simultaneous determination of 7 kinds of illegally-added chemical drugs,including Carbamazepine,Chlorpromazine,Sulpiride,Oxcarbazepine,Olanzapine,Doxepin and Quetiapine,in improving sleep quality health food by high performance liquid chromatography(HPLC).Methods A C1s column (250 mm × 4.6 mm,5 μm) was used for chromatographic separation,which was eluted with methanol-0.02 mol/L potassium dihydrogen phosphate as mobile phase at 35 ℃.The diode array detector was used for detection at 250 nm.Results The 7 chemical drugs showed a linear relationship in the range of 20-600 μg/ml.The limit of determination and the limit of quantification of the method were in the ranges of 0.4 -2.5 ng/g and 2.5-5.0 ng/g,respectively.The RSD of repeatability was in the range of 0.1％-3.6％ and the precision of instrument was 0.2％-2.8％.The average recovery rate was 95％-102％.The above seven components solution was stable in 32 h.Conclusion This method is simple and convenient,and suitable for simultaneous rapid determination of 7 kinds of illegally-added chemical drugs in health food.关键词
改善睡眠类保健食品/HPLC/非法添加化学药品Key words
improving sleep quality health food/high performance liquid chromatography/illegally-added chemical drugs分类
医药卫生
