山西医科大学学报2017，Vol.48Issue(11)：1172-1176,5.DOI:10.13753/j.issn.1007-6611.2017.11.018
结直肠癌组织SHP-2和STAT3蛋白表达及临床意义
Expression and clinical significance of SHP-2 and STAT3 in colorectal carcinoma
摘要
Abstract
Objective To investigate the expression of STAT3 protein and SHP-2 protein in colorectal carcinoma(CRC),and to explore its relationships with clinical characteristics.Methods Immunohistochemisty was used to detect the expression of STAT3 and SHP-2 in 168 cases of CRC tissues and 40 cases of normal tissues.The expression of STAT3 and SHP-2 was compared between patients with different clinicopathological features of CRC.The expression of STAT3 and SHP-2 in 40 cases of CRC tissues and matched adja cent non-tumor tissues was detected by Western-blot.Results The results of immunohistochemisty demonstrated that the positive expression rate of STAT3 protein in 168 cases of CRC was significantly higher than that of normal tissues (P ＜ 0.05),but the positive expression rate of SHP-2 protein in 168 cases of CRC was significantly lower than that of normal tissues (P ＜ 0.05).Western-blot results showed that STAT3 protein in CRC was higher than that of matched adjacent non-tumor tissues (0.421 3 ± 0.112 7 vs 0.301 7 ± 0.079 9,P ＜ 0.05),but SHP-2 protein level in CRC was lower than that of matched adjacent non-tumor tissues (0.239 6 ± 0.065 5 vs 0.766 5 ± 0.113 3,P ＜ 0.001).The expression of STAT3 in CRC tissues was correlated with differentiation grade,lymph node metastasis,distant metastasis and TNM stage,but not related to gender,age and invasion depth.The expression of SHP-2 in CRC tissues was correlated with differentiation grade and lymph node metastasis,but not related to gender,age,invasion depth,distant metastasis and TNM stage.The expression of STAT3 protein was negatively correlated with the expression of SHP-2 in colorectal carcinoma protein (r =-0.501,P ＜ 0.05).Conclusion The expression of STAT3 is higher but SHP-2 is lower in CRC patients,suggesting that the two proteins may be involved in the evolution and development process of CRC,which may be used as potential therapeutic targets in the treatment.关键词
结直肠癌/STAT3/SHP-2/免疫组织化学/蛋白免疫印迹Key words
colorectal carcinoma/STAT3/SHP-2/immunohistochemistry/Western-blot分类
医药卫生引用本文复制引用
谭婉燕,马清峰..结直肠癌组织SHP-2和STAT3蛋白表达及临床意义[J].山西医科大学学报,2017,48(11):1172-1176,5.基金项目
湖北省自然科学基金资助项目(2014CFB434) （2014CFB434）
