生殖医学杂志2017，Vol.26Issue(12)：1177-1181,5.DOI:10.3969/j.issn.1004-3845.2017.12.003
亚甲基四氢叶酸还原酶基因C677T多态性与男性不育的相关性及机制研究
Correlation between MTHFR C677T polymorphism and sperm indexes in infertile males
摘要
Abstract
Objective:To explore relationship between MTHFR C677T polymorphism and sperm indexes of infertile males.Methods:The clinical data of 59 male infertile patients in our hospital from January 2016 to March 2017 were retrospective analyzed.The patients were divided into three groups according to MTHFR gene C677T genotype(CC,CT and TT group).The sperm indexes including motility,viability,DNA integrity,acrosome integrity and ROS level were compared among the groups.Results:The proportion of normal sperm in CT genotype group was significantly lower than that in CC genotype group (P＜0.05).The proportion of severe oligozoospermia,asthenospermia and severe oligoasthenospermia in TT genotype group was significantly higher than that in CC genotype group,and the proportion of severe oligoasthenospermia in TT genotype group was significantly higher than that of CT group(P＜0.05).The proportion of normal sperm in TT genotype group was significantly lower than that of CC and CT genotype group(P＜0.05).The sperm viability in CT genotype group was significantly lower than that in CC genotype group,and the sperm viability in TT genotype group was significantly lower than that in CC and CT genotype(P＜0.05).The sperm DNA integrity related indicators in TT genotype group was significantly higher than that inCCandCTgenotype(P＜0.05).The spermacrosome integrity in TT genotype group was significantly lower than that in CC and CT genotype(P＜0.05).The ROS level of CT genotype group was significantly higher than that of CC genotype group(P＜0.05).The ROS level of TT genotype group was significantly higher than that of CC and CT genotype group(P＜0.05).Conclusions:MTHFR C677T polymorphism is related with sperm index of infertile males,which might be related to the ROS level.关键词
亚甲基四氢叶酸还原酶/基因多态性/活性氧Key words
Methylene tetrahydrofolate reductase/Gene polymorphism/Reactive oxygen species引用本文复制引用
邹宇洁,常硕,庞湘力,龙文,尹太郎,杨菁..亚甲基四氢叶酸还原酶基因C677T多态性与男性不育的相关性及机制研究[J].生殖医学杂志,2017,26(12):1177-1181,5.基金项目
湖北省卫计委面上项目(WJ2017M011) （WJ2017M011）
湖北省自然科学基金(2016CFB352) （2016CFB352）
中国国家留学基金(201706275124) （201706275124）
