天然产物研究与开发2017，Vol.29Issue(11)：1813-1817,5.DOI:10.16333/j.1001-6880.2017.11.001
蜂毒肽对成熟树突细胞诱导T细胞分化的免疫调节的影响
Immune Regulation Influence of Melittin on T Cells Induced Differentiation by Mature Dendritic Cells
摘要
Abstract
To study the intervention of melittin (MT) on dendritic cells (DC) co-cultured with lymphocytes stimulated by lipopolysaccharide (LPS),the impact of MT on the differentiation of Th1/Th2 was investigated.MTS was used to detect the activity of DC co-cultured with lymphocytes by LPS and MT;Flow cytometry was used to detect the ratio of Th1 and Th2 in the co-culture of DCs stimulated by MT and LPS;ELISA was used to detect the concentration IL-4,INF-γin cell supernatant of MT and LPS stimulated co-cultured DC and lymphocyte.The results showed that a certain concentration of MT and LPS can stimulate the co-cultured activity of DC with lymphocytes.In the induction of mature DC,MT can increase the Th1/Th2 ratio,the Th1 percentage (P ＜ 0.05) and the IFN-γ (P ＜ 0.01).But it had no effect on the concentration of IL-4 and Th2 (P ＞ 0.05).The study demonstrated that through upregulating the expression of Th1 cell ratio and IFN-γconcentration,MT promoted the differentiation of Th1/Th2 cells to the Th1 pathway,thus playing immunomodulatory effects on T cells.关键词
蜂毒肽/树突细胞/T细胞/免疫调节Key words
melittin/dendritic cell/T cell/immune regulation分类
医药卫生引用本文复制引用
熊浪平,贺守第,关彤,莫丽华,杨平常,刘志刚,吉琼梅,黄胜光..蜂毒肽对成熟树突细胞诱导T细胞分化的免疫调节的影响[J].天然产物研究与开发,2017,29(11):1813-1817,5.基金项目
国家自然科学基金(81271950) （81271950）
深圳市科技计划(JCYJ20130402151227168 （）
JCYJ20150402152130192 （）
005177) （）
深圳市南山区科技项目(2014046) （2014046）
