天然产物研究与开发2017，Vol.29Issue(11)：1818-1823,6.DOI:10.16333/j.1001-6880.2017.11.002
人参皂苷Re与精氨酸美拉德反应产物的抗氧化性研究
Antioxidant Activity of Maillard Reaction Products Prepared from Ginsenoside Re with L-Arginine
摘要
Abstract
The aim of the present study was to evaluate the important role of ginsenoside Re-arginine Maillard reaction products (MRPs) in the free radical scavenging and against Cu2 +-induced oxidative stress in HepG2 cells.Ginsenoside Re with arginine was heated at 100 ℃ for 4 h to prepare the MRPs.The MRPs was analyzed by HPLC,the browning level was assayed at 420 nm,total phenol content was assayed by the Folin-Ciocalteu method and the result was illustrated as milligrams of GAE per gram of each sample tested.The change of Cu2+ chelating activities and the scavenging effect on DPPH radical were also evaluated.Meanwhile,the antioxidant ability was also assayed in HepG2 cells by using the Cu2+-induced oxidative stress model.The results indicate that the ginsenoside Re is transformed into less-polar ginsenosides such as Rg2,Rg6 and F4 during Maillard reaction,and the glucose molecule at carbon-20 is separated,the browning level and the total phenol increase obviously,the Cu2+ chelating activity and DPPH radical scavenging activity are enhanced significantly.Comparing to the control,there is a 3.16-fold increase of oxidative stress of HepG2 cells under the influence of Cu2 +.The oxidative stress induced by Cu2 + is only marginally inhibited by the treatment of ginsenoside Re or Re-arginine mixture (not significant,p ＞ 0.05) but significantly reduced by the treatment with Re-arginine MRPs in the concentration range of 100 to 300 μg/mL.Because of their high cell-membrane permeability,the ginsenoside Rearginine MRPs increase the free radical-scavenging acivity in HepG2 cells,showing cellular antioxidant activity against Cu2+-induced oxidative stress higher than that of Re.关键词
人参皂苷Re/精氨酸/美拉德反应产物/抗氧化Key words
ginsenoside Re/arginine/Maillard reaction products/antioxidant分类
医药卫生引用本文复制引用
张儒,张变玲,易婷,贺婷,刘心怡..人参皂苷Re与精氨酸美拉德反应产物的抗氧化性研究[J].天然产物研究与开发,2017,29(11):1818-1823,6.基金项目
湖南省自然科学基金(2016JJ2037,2017JJ3048) （2016JJ2037,2017JJ3048）
湖南省教育厅基金(16C0394) （16C0394）
中国博士后特别资助和面上资助项目(2017T100601,2016M590746) （2017T100601,2016M590746）
国家级大学生创新创业训练计划(201411342004) （201411342004）
