天然产物研究与开发2017，Vol.29Issue(11)：1846-1850,1927,6.DOI:10.16333/j.1001-6880.2017.11.006
马齿苋总黄酮对Aβ25-35阿尔茨海默症小鼠学习记忆功能的影响
Effects of Total Flavone in Portulacaoleracea L.on Learning and Memory of Alzheimer's Disease Mice Caused by Aβ25-35
摘要
Abstract
This study was designed to investigate the effects of total flavone in Portulacaoleracea L.(POTF) on learning and memory of mice with Alzheimer's disease (AD) caused by Aβ25-35.Sixty mice were divided randomly into six groups:sham operation group,model group,high,medium and low dose of POTF treatment groups.AD mice were established by hippocampal injection of Aβ25-35.Different concentrations of POTF were treated for 30 days.Morris water maze was used to test the spatial learning and memory ability in each group.Hydroxylamine and spectrophotometer were used for the activities of Ach and AchE in hippocampus,respectively.Expression of CREB mRNA and protein was detected by qPCR and Western blot.The results showed that compared with sham group,the spatial learning and memory in model group was significantly decreased (P ＜ 0.01),the levels of Ach,AchE and CREB were also decreased (P ＜ 0.05).Compared with model group,mice treated with high dose of POTF showed much less latency (P ＜0.01) and increased times across platform (P ＜ 0.01) in Morris water maze,and higher levels of Ach,AchE and CREB mRNA and protein in hippocampus in different degree (P ＜0.05 or P ＜0.01).In conclusion,POTF has a protective effect on the learning and memory of AD mice by enhancing the metabolism of Ach and CREB pathway in hippocampus.关键词
马齿苋/总黄酮/阿尔茨海默症/学习记忆Key words
Portulacaoleracea L./total flavone/Alzheimer's disease/learning and memory分类
医药卫生引用本文复制引用
勾洵,黄思莹,姚雪梅,赵溪岩,张尔亮..马齿苋总黄酮对Aβ25-35阿尔茨海默症小鼠学习记忆功能的影响[J].天然产物研究与开发,2017,29(11):1846-1850,1927,6.基金项目
四川省教育厅科研项目(14ZB0033) （14ZB0033）
四川师范大学实验技术与管理项目(SYJS2017028) （SYJS2017028）
海南医学院科研培育基金(HY2016-13) （HY2016-13）
