天然产物研究与开发2017，Vol.29Issue(11)：1905-1909,5.DOI:10.16333/j.1001-6880.2017.11.016
高效液相色谱法同时测定青钱柳中9种指标成分的含量
Simultaneous Determination of Nine Chemical Markers of Cyclocaryae Folium by HPLC
摘要
Abstract
HPLC method was developed for the simultaneous determination of chlorogenic acid,vanililic acid,ellagic acid,sioquercitin,afzelin,quercetin,kaempferol,kaempferidel and ursolic acid in Cyclocaryae Folium.The ZORBAX Eclipse XDB-C18column (150 mm × 4.6 mm,3.5 μm) was used with 0.2％ phosphoric acid in acetonitrile (A) and 0.2％ phosphoric acid in water (B) as mobile phases in gradient elution mode.The flow rate was 0.7 mL/min,the column temperature was 35 ℃.The DAD detection wavelength was set at 210 nm for vanililic acid,ellagic acid and ursolic acid,360 nm for the others.As a result,nine chemical markers were separated well,the average recoveries were between 95.86-103.30％,RSD ＜ 2.5％.The developed HPLC method was simple,accurate and reproductive.It is suitable for the determination of nine chemical markers of Cyclocaryae Folium.关键词
青钱柳/高效液相色谱/多指标成分/不同产地/含量测定Key words
Cyclocaryae Folium/HPLC/multiple chemical marks/content determination分类
医药卫生引用本文复制引用
汪荣斌,秦亚东,陈颖,王存琴,李林华,周守标..高效液相色谱法同时测定青钱柳中9种指标成分的含量[J].天然产物研究与开发,2017,29(11):1905-1909,5.基金项目
安徽省卫生计生委中医药科研课题(2014zy45) （2014zy45）
安徽省高校优秀青年人才支持计划(gxyq2017209) （gxyq2017209）
安徽高校自然科学研究重点项目(KJ2015A343) （KJ2015A343）
安徽省高校学科(专业)拔尖人才学术资助重点项目(gxbjZD2016106) （专业）
